Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KU525_RS03210 Genome accession   NZ_CP077920
Coordinates   649338..650702 (-) Length   454 a.a.
NCBI ID   WP_049307770.1    Uniprot ID   A0AAN2D677
Organism   Staphylococcus aureus strain 277     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 644338..655702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU525_RS03195 (KU525_03180) cysE 645159..645806 (-) 648 WP_001805237.1 serine O-acetyltransferase -
  KU525_RS03200 (KU525_03185) gltX 646229..647683 (-) 1455 WP_001283792.1 glutamate--tRNA ligase -
  KU525_RS03205 (KU525_03190) - 648240..649313 (-) 1074 WP_001028376.1 PIN/TRAM domain-containing protein -
  KU525_RS03210 (KU525_03195) radA 649338..650702 (-) 1365 WP_049307770.1 DNA repair protein RadA Machinery gene
  KU525_RS03215 (KU525_03200) - 651187..653643 (-) 2457 WP_000897132.1 ATP-dependent Clp protease ATP-binding subunit -
  KU525_RS03220 (KU525_03205) - 653657..654664 (-) 1008 WP_000149503.1 protein arginine kinase -
  KU525_RS03225 (KU525_03210) - 654654..655220 (-) 567 WP_000882069.1 UvrB/UvrC motif-containing protein -
  KU525_RS03230 (KU525_03215) - 655239..655700 (-) 462 WP_000551762.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49835.40 Da        Isoelectric Point: 7.2135

>NTDB_id=585110 KU525_RS03210 WP_049307770.1 649338..650702(-) (radA) [Staphylococcus aureus strain 277]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVKEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=585110 KU525_RS03210 WP_049307770.1 649338..650702(-) (radA) [Staphylococcus aureus strain 277]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCTAATTG
TGGCGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCAGCCAATCCTAAACATGGAGTTAAAACCAAGGAATTAGCAG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACAACGCCGAGAGTGTTAACAGATTCAGCAGAATTCAACCGTGTA
TTAGGTGGAGGTATTGTGAGCGGATCGTTAGTACTTATTGGTGGGGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCATCGTTATCTCAAAAGAAAAAAGTACTATATATTACTGGAGAAGAATCGCTTAGTCAGACTAAATTACGTG
CAGAGCGATTAGATGAAGATTCAAGTGAATTGCAAGTATTAGCTGAAACAGATCTTGAAGTTATTTATCAAACAGTAAAA
GAAGAACAACCTGATTTATTAGTAGTGGATTCGATTCAAACAATATATCATCCTGAAATCAGCTCTGCGCCAGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAATATTGCTAAACAAATGAACATTGCAACTTTTATAGTGGGTCATG
TAACGAAAGAAGGTCAAATTGCTGGCCCAAGATTGCTAGAACACATGGTTGATACTGTGCTTTATTTTGAAGGCGATGAA
CACCACGCATATCGAATTTTGCGAGCTGTTAAAAACCGTTTTGGTTCAACGAATGAAATGGGAATCTTCGAAATGAAGCA
AAGTGGATTAAAAGGTGTAAATAATCCATCTGAAATGTTTTTAGAAGAACGTTCAACAAATGTTCCAGGTTCAACAATTG
TTGCAACCATGGAGGGAACCAGACCACTTTTAATAGAAGTTCAAGCGCTGGTAACTCCAACGACTTTTAACAATCCGAGA
CGAATGGCAACAGGGATTGATCATAATCGATTAAGTTTGTTGATGGCTGTTTTGGAAAAGAAAGAAAATTATCTATTACA
ACAACAAGATGCTTATATCAAAGTAGCTGGCGGTGTAAAGTTAACGGAGCCAGCAGTTGATTTAAGTGTAATTGTAGCAA
CTGCATCTAGCTTTAAAGATAAAGCTGTCGACGGATTAGATTGCTATATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGTGTATCTCGGATAGAACAACGCGTGAAAGAGGCTGCAAAACTAGGTTTCAAACGTGTAATTATTCCTAAAAATAATAT
AGGCGGATGGACATATCCTGAAGGTATACAAGTAATAGGTGTAACTACTGTACATGAAGCATTGTCATTTGCTCTTCATT
CATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.885

100

0.676
  radA Streptococcus pneumoniae Rx1

62.054

98.678

0.612
  radA Streptococcus pneumoniae D39

62.054

98.678

0.612
  radA Streptococcus pneumoniae R6

62.054

98.678

0.612
  radA Streptococcus pneumoniae TIGR4

62.054

98.678

0.612
  radA Streptococcus mitis NCTC 12261

62.054

98.678

0.612
  radA Streptococcus mitis SK321

62.054

98.678

0.612