Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KU501_RS08015 Genome accession   NZ_CP077911
Coordinates   1665041..1666015 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 318     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1660041..1671015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU501_RS07975 (KU501_07920) gcvT 1660542..1661633 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  KU501_RS07980 (KU501_07925) - 1661792..1662316 (-) 525 WP_001015125.1 shikimate kinase -
  KU501_RS07985 (KU501_07930) - 1662306..1662452 (-) 147 WP_001789879.1 hypothetical protein -
  KU501_RS07990 (KU501_07935) comGF 1662549..1663046 (-) 498 WP_001794055.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU501_RS07995 (KU501_07940) comGE 1662964..1663263 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU501_RS08000 (KU501_07945) comGD 1663250..1663696 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU501_RS08005 (KU501_07950) comGC 1663674..1663985 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  KU501_RS08010 (KU501_07955) comGB 1663999..1665069 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU501_RS08015 (KU501_07960) comGA 1665041..1666015 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU501_RS08020 (KU501_07965) - 1666067..1666690 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KU501_RS08025 (KU501_07970) - 1666687..1667016 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KU501_RS08030 (KU501_07975) - 1667016..1668002 (-) 987 WP_225798517.1 ROK family glucokinase -
  KU501_RS08035 (KU501_07980) - 1667999..1668202 (-) 204 WP_000087561.1 YqgQ family protein -
  KU501_RS08040 (KU501_07985) - 1668183..1669646 (-) 1464 WP_001017873.1 rhomboid family protein -
  KU501_RS08045 (KU501_07990) - 1669658..1670197 (-) 540 WP_000164553.1 5-formyltetrahydrofolate cyclo-ligase -
  KU501_RS08050 (KU501_07995) rpmG 1670406..1670555 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=584913 KU501_RS08015 WP_000697220.1 1665041..1666015(-) (comGA) [Staphylococcus aureus strain 318]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=584913 KU501_RS08015 WP_000697220.1 1665041..1666015(-) (comGA) [Staphylococcus aureus strain 318]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTGCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1