Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   MW399_RS04035 Genome accession   NZ_CP095540
Coordinates   860971..862968 (-) Length   665 a.a.
NCBI ID   WP_001088086.1    Uniprot ID   -
Organism   Escherichia coli strain GN03204     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 855971..867968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW399_RS04010 (MW399_04010) arcC 856302..857258 (+) 957 WP_000240546.1 carbamate kinase -
  MW399_RS04015 (MW399_04015) - 857275..857664 (+) 390 WP_000214035.1 glutamyl-tRNA amidotransferase -
  MW399_RS04020 (MW399_04020) - 857690..858913 (+) 1224 WP_001227356.1 cytosine permease -
  MW399_RS04025 (MW399_04025) - 858991..859896 (+) 906 WP_000470720.1 LysR substrate-binding domain-containing protein -
  MW399_RS04030 (MW399_04030) - 860096..860941 (+) 846 WP_001060029.1 MurR/RpiR family transcriptional regulator -
  MW399_RS04035 (MW399_04035) recP/tkt 860971..862968 (-) 1998 WP_001088086.1 transketolase Machinery gene
  MW399_RS04040 (MW399_04040) ptsG 863019..864527 (-) 1509 WP_000037971.1 glucose-specific PTS transporter subunit IIBC -
  MW399_RS04045 (MW399_04045) hxlB 864569..865126 (-) 558 WP_000339043.1 6-phospho-3-hexuloisomerase -
  MW399_RS04050 (MW399_04050) metE 865465..867726 (-) 2262 WP_000153956.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 72988.66 Da        Isoelectric Point: 5.7465

>NTDB_id=583983 MW399_RS04035 WP_001088086.1 860971..862968(-) (recP/tkt) [Escherichia coli strain GN03204]
MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHLRHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYD
LSMDDIRDFRQLHSRTPGHPEYGYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGHDPEAVDAAVREAKSVTDKPSLL
CCKTIIGFGSPNKANSHDCHGSALGADEVALVRERLQWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPEL
AAEFTRRMKGDLPAGWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKSVDFTGENP
AGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAALMKIRSVFVYTHDTIGLGEDGPTHQPVEQLA
SLRLTPNMETWRGCDQVEVAVAWQQAIERKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSE
IELVVSAAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVGLDGKVIGMTSFGESAP
ANVLFKHFGFTPENVLAQARELLNS

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=583983 MW399_RS04035 WP_001088086.1 860971..862968(-) (recP/tkt) [Escherichia coli strain GN03204]
ATGAATTCACAATTACTGGCTAATGCCATCCGAATGTTAAGTGTTGATGCGATCCAGAAAGCGAACTCCGGTCATCCGGG
CGCGCCGATGGGGATGGCGGATATTGCCGAAGTGGTCTGGCGTCGTCACCTGCGTCATAACCCAAAAAATCCGCAGTGGT
TTAACCGCGACCGCTATGTTCAGTCGAACGGTCACGGCTCCATGTTGATTTACGCTTTGCTGCATCTTACAGGCTACGAC
CTGTCGATGGATGATATTCGCGACTTTCGTCAGCTCCACTCCCGCACGCCGGGTCATCCAGAATATGGTTATACGCCGGG
TGTTGAAACCACCACCGGCCCGTTGGGGCAGGGCGTAGCTAATGCGGTGGGGATGGCGATTGCGGAGAAGGCGCTGGCGG
CAGAATTTAACAAACCGGGCTTTAATATTGTTGATCATCACACATGGTTATTTTTGGGTGATGGCTGTCTGATGGAAGGG
ATCTCCCACGAGGCCTGTGGTCTTGCAGGAACGCTGAAGCTCGGCAATCTGATTGCTATCTGGGACGATAACGGTATTTC
GATCGATGGTCACGTAGAAGGCTGGTTCGCTGAAGATACGGCTGCCCGTTTTCGTGCCTACGGCTGGCATGTCATTGAAG
GCGTTGATGGTCACGATCCAGAAGCGGTAGATGCTGCGGTGCGTGAAGCGAAAAGCGTCACGGATAAGCCCAGTCTGCTG
TGCTGCAAAACGATTATCGGTTTTGGTTCGCCAAATAAAGCCAACAGCCACGATTGCCACGGCTCGGCGCTGGGTGCAGA
TGAAGTGGCGCTGGTGCGTGAACGTCTGCAATGGCCGTATGCCCCTTTCGAAATTCCTGGTGAAATTTATGCCGAATGGG
ATGCGACCGAGAAAGGCGCGCAGGTTCAACAGGAATGGGATGCACTGTTTGCCGACTACGCGAAGCAGTGGCCGGAACTG
GCGGCGGAGTTTACTCGTCGCATGAAGGGTGATTTGCCTGCTGGCTGGGTCGAGAATATGCAGAAATATGTGCACGATCT
GCAATCCCATCCGGCGGCGCTGGCAACCCGTCAGGTTAGCCAGAAATGCCTCAATCACTTCGCAGATATGCTGCCGGAAC
TGATGGGCGGCTCGGCGGACTTGTCGCCCTCTAACCTGACTCGCCATCAGAAATCGGTTGATTTCACCGGAGAAAATCCG
GCGGGGAATTACATCTCCTATGGCGTGCGTGAATTTGGTATGTCGGCCATCATGAACGGTCTTGCGCTGCATGGTGGGTT
TATCCCTTACGGCGGTACTTTCCTGATGTTTATGGAATATGCGCGTAATGCACTGCGCATGGCGGCGCTGATGAAGATCC
GCTCGGTGTTTGTTTATACCCACGATACCATCGGCCTGGGCGAAGACGGCCCGACACATCAGCCCGTTGAGCAACTGGCG
TCGCTGCGTCTGACGCCGAATATGGAAACCTGGCGCGGTTGCGATCAGGTGGAAGTGGCGGTGGCCTGGCAACAGGCGAT
TGAGCGCAAAGATGGCCCAACGTCGTTGGTATTAACGCGCCAACCTCTGGCGCAACAGCCGCGCACCGCTGCACAGTTGG
CAGAGATTGCGCGTGGTGGTTATGTACTGAGTGATTGTGACGGTCAGCCTGAGATGATCCTCATTTCTGCGGGTTCAGAG
ATTGAGTTAGTGGTCAGCGCCGCAAAAGCGTTAACGGAAGAAGGGCGTAAAGTTCGCGTGGTTTCCATGCCGTGTACCGA
GCGTTTCGATAATCAGGATGCGGCGTACAAAGAGTCGGTATTGCCAAAAGCTGTACGCAAACGTCTGGCGGTAGAAGCCA
GCATCGCCGGATTCTGGGAACGCTACGTGGGCCTGGACGGTAAAGTGATCGGCATGACGAGCTTTGGTGAGTCTGCTCCG
GCGAATGTGTTGTTCAAACACTTTGGTTTTACGCCGGAAAATGTCCTGGCGCAGGCGCGTGAGCTACTGAACAGCTAA

Domains

Predicted by InterProScan.

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-333)

Transketolase, C-terminal domain (Transketolase_C)

(540-655)

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.041

99.098

0.466