Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   MW401_RS10635 Genome accession   NZ_CP095510
Coordinates   2249307..2250734 (-) Length   475 a.a.
NCBI ID   WP_000980589.1    Uniprot ID   P04995
Organism   Escherichia coli strain GN02270     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2244307..2255734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW401_RS10605 (MW401_10600) yeeZ 2244362..2245186 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  MW401_RS10610 (MW401_10605) yeeY 2245232..2246161 (+) 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
  MW401_RS10615 (MW401_10610) yoeI 2246376..2246438 (+) 63 WP_010723108.1 membrane protein YoeI -
  MW401_RS10620 (MW401_10615) plaP 2246428..2247786 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  MW401_RS10625 (MW401_10620) tsuA 2247965..2249023 (+) 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
  MW401_RS10630 (MW401_10625) tsuB 2249037..2249264 (+) 228 WP_000234895.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  MW401_RS10635 (MW401_10630) sbcB 2249307..2250734 (-) 1428 WP_000980589.1 exodeoxyribonuclease I Machinery gene
  MW401_RS10640 (MW401_10635) dacD 2250943..2252109 (+) 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  MW401_RS10645 (MW401_10640) sbmC 2252228..2252701 (+) 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
  MW401_RS10650 (MW401_10645) yeeA 2252899..2253957 (+) 1059 WP_001200891.1 FUSC family protein -
  MW401_RS10655 (MW401_10650) tmaR 2254129..2254458 (+) 330 WP_000450409.1 DUF496 family protein -
  MW401_RS26240 - 2254559..2254741 (-) 183 WP_001016348.1 EutP/PduV family microcompartment system protein -
  MW401_RS26360 - 2254813..2254941 (+) 129 Protein_2088 transposase domain-containing protein -
  MW401_RS10670 (MW401_10665) - 2255230..2255322 (-) 93 Protein_2089 DUF957 domain-containing protein -
  MW401_RS10675 (MW401_10670) yeeW 2255356..2255550 (-) 195 WP_000988600.1 DUF5983 family protein -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54500.81 Da        Isoelectric Point: 5.4700

>NTDB_id=583342 MW401_RS10635 WP_000980589.1 2249307..2250734(-) (sbcB) [Escherichia coli strain GN02270]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=583342 MW401_RS10635 WP_000980589.1 2249307..2250734(-) (sbcB) [Escherichia coli strain GN02270]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CTGATGACTATTTACCCCAGCCAGGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTGCACGTTTCCGGAATGTTTGGAGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAAAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGT
GGTGGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTG
ACGCAGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGGATTGAAAAGCTGTTGTTCAATTATCGGGCACGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGTCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGTTATGCTGATGAATTGCAGATGCTGGTACAACAAT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCACTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(13-188)

Exonuclease C-terminal (ExoI_SH3)

(212-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1FXX
  PDB 2QXF
  PDB 3C94
  PDB 3C95
  PDB 3HL8
  PDB 3HP9
  PDB 4HCB
  PDB 4HCC
  PDB 4JRP
  PDB 4JRQ
  PDB 4JS4
  PDB 4JS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

98.947

0.585