Detailed information NTG0058325

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AVCA_RS05440
• Genome accession: NC_021149
• Coordinates: 1159711..1160580 (-)
• Length: 289 a.a.
• NCBI ID: WP_012699852.1
• Uniprot ID: M9Y9L4
• Organism: Azotobacter vinelandii CA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1154711..1165580

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AVCA_RS05410 (AvCA_12000)	-	1155225..1156523 (-)	1299	WP_012699846.1	NAD(P)/FAD-dependent oxidoreductase	-
AVCA_RS24420 (AvCA_12010)	-	1156588..1156761 (-)	174	WP_012699847.1	DUF3094 domain-containing protein	-
AVCA_RS05415 (AvCA_12020)	-	1157066..1157491 (+)	426	WP_012699848.1	GNAT family N-acetyltransferase	-
AVCA_RS05420 (AvCA_12030)	-	1157488..1157955 (+)	468	WP_012699849.1	hypothetical protein	-
AVCA_RS05425 (AvCA_12040)	-	1157952..1158638 (+)	687	WP_012699850.1	energy-coupling factor ABC transporter permease	-
AVCA_RS05430	yacG	1158744..1158944 (-)	201	WP_041806982.1	DNA gyrase inhibitor YacG	-
AVCA_RS05435 (AvCA_12060)	coaE	1158941..1159549 (-)	609	WP_012699851.1	dephospho-CoA kinase	-
AVCA_RS05440 (AvCA_12070)	pilD	1159711..1160580 (-)	870	WP_012699852.1	prepilin peptidase	Machinery gene
AVCA_RS05445 (AvCA_12080)	pilC	1160583..1161800 (-)	1218	WP_012699853.1	type II secretion system F family protein	Machinery gene
AVCA_RS05450 (AvCA_12090)	pilB	1162025..1163725 (-)	1701	WP_012699854.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AVCA_RS24425 (AvCA_12104)	-	1164254..1164751 (+)	498	WP_012699856.1	pilin	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31650.64 Da
• Isoelectric Point: 6.9531

>NTDB_id=58325 AVCA_RS05440 WP_012699852.1 1159711..1160580(-) (pilD) [Azotobacter vinelandii CA]
MSIVDLLAGPGLAFAVFAGLLGLLVGSFLNVVVHRLPKMMLHDWQAQAREVLELPNEPPGEPFNLLLPHSHCPHCGHAIR
PWENIPLLSWLLLGGRCSACKARIGARYPLVELTCGLLSAHVAWHYGFGWQAGAMLLLGWALLAMSLIDYDHQLLPDSLV
LPLLWLGLILNSFSLFVSLEDALWGAVTGYLSLWSVYWLFKLFTGKEGMGYGDFKLLAMLGAWGGWQILPLTVLLSSLVG
AMLGIVILRLRKAESGTPIPFGPFLAFAGWIALLWGEQITAGYLRFAGL

Nucleotide

• Length: 870 bp

>NTDB_id=58325 AVCA_RS05440 WP_012699852.1 1159711..1160580(-) (pilD) [Azotobacter vinelandii CA]
ATGTCCATCGTCGATCTTCTGGCCGGCCCCGGACTGGCCTTCGCCGTTTTCGCGGGGCTGCTGGGCCTGCTGGTCGGCAG
CTTTCTCAACGTGGTCGTCCACCGCCTGCCGAAGATGATGCTGCACGACTGGCAGGCACAGGCGCGGGAAGTGCTGGAAC
TGCCCAACGAGCCTCCCGGCGAGCCTTTCAATCTGCTGCTGCCCCACTCCCATTGCCCACATTGCGGGCACGCGATCAGG
CCCTGGGAGAACATCCCCCTGCTCAGCTGGCTGCTCCTGGGTGGCCGCTGCTCGGCCTGCAAGGCCCGGATCGGCGCGCG
CTACCCCCTGGTGGAGCTGACCTGCGGGCTGCTTTCGGCCCATGTCGCCTGGCATTACGGCTTCGGCTGGCAAGCCGGCG
CCATGTTGTTGCTCGGCTGGGCTCTGCTGGCCATGAGCCTGATCGACTACGATCATCAACTGCTGCCGGATTCGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCCTCAACAGCTTCAGTCTGTTCGTTTCCCTGGAGGACGCCCTCTGGGGCGCGGT
GACCGGCTACCTCAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGTTCACCGGCAAGGAAGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCGATGCTCGGCGCCTGGGGTGGCTGGCAGATCCTGCCGCTGACCGTACTGCTCTCCTCGCTGGTCGGC
GCCATGCTGGGGATCGTCATCCTGCGCCTGCGCAAGGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTTTCTCGCCTT
CGCCGGCTGGATCGCCCTGCTCTGGGGCGAGCAGATCACCGCCGGCTACCTGCGCTTCGCCGGTCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: M9Y9L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.934	94.81	0.54
pilD	Vibrio campbellii strain DS40M4	53.114	94.464	0.502
pilD	Acinetobacter nosocomialis M2	50	94.81	0.474
pilD	Acinetobacter baumannii D1279779	50	94.81	0.474
pilD	Neisseria gonorrhoeae MS11	48.134	92.734	0.446