Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   MWN28_RS07455 Genome accession   NZ_CP095448
Coordinates   1543295..1545298 (-) Length   667 a.a.
NCBI ID   WP_000087280.1    Uniprot ID   P33570
Organism   Escherichia coli strain LS45     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1538295..1550298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN28_RS07440 (MWN28_07440) aegA 1539401..1541380 (+) 1980 WP_001078911.1 formate-dependent uric acid utilization protein AegA -
  MWN28_RS07445 (MWN28_07445) nudK 1541448..1542023 (+) 576 WP_001330582.1 GDP-mannose pyrophosphatase NudK -
  MWN28_RS07450 (MWN28_07450) ypfG 1542149..1543192 (+) 1044 WP_001270513.1 DUF1176 domain-containing protein -
  MWN28_RS07455 (MWN28_07455) recP/tkt 1543295..1545298 (-) 2004 WP_000087280.1 transketolase Machinery gene
  MWN28_RS07460 (MWN28_07460) tal 1545318..1546268 (-) 951 WP_001003709.1 transaldolase -
  MWN28_RS07465 (MWN28_07465) maeB 1546557..1548836 (+) 2280 WP_000342643.1 NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase -
  MWN28_RS07470 (MWN28_07470) eutS 1549129..1549464 (+) 336 WP_000356956.1 ethanolamine utilization microcompartment protein EutS -
  MWN28_RS07475 (MWN28_07475) eutP 1549477..1549956 (+) 480 WP_072754469.1 ethanolamine utilization acetate kinase EutP -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 73042.72 Da        Isoelectric Point: 6.2536

>NTDB_id=582918 MWN28_RS07455 WP_000087280.1 1543295..1545298(-) (recP/tkt) [Escherichia coli strain LS45]
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=582918 MWN28_RS07455 WP_000087280.1 1543295..1545298(-) (recP/tkt) [Escherichia coli strain LS45]
ATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAATTCTGGTCATCCCGG
CGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAATCCTACCGACCCAACCTGGT
ATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTGCATCTGACCGGTTACGAC
CTGCCGCTGGAAGAACTGAAGAACTTTCGTCAGTTGCATTCGAAAACCCCTGGTCACCCGGAAATCGGCTATACGCCTGG
TGTTGAAACCACCACCGGTCCACTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACGCTGGCGG
CGCAGTTTAACCAGCCAGATCATGAAATTGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGT
ATTTCCCACGAAGTCTGTTCGCTGGCGGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTC
CATCGACGGTGAAACCGAAGGCTGGTTTACCGACGATACAGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCACG
AGATCGACGGCCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATT
ATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGA
AGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGG
ATGCCCGCGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTG
GCAGAAGAGTTTACCCGTCGGATGAGCGGTGGTTTACCGAAGGACTGGGAGAAAACGACTCAGAAATATATCAATGAGTT
GCAGGCGAATCCGGCGAAAATCGCTACCCGTAAGGCTTCGCAAAATACGCTTAACGCTTACGGGCCTATGCTACCGGAGC
TGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAGGTTCTGTTTCGCTGAAGGAAGATCCGGCG
GGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATTGCGCACCACGGCGGCTTTGT
GCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGC
AGATTATGGTTTATACCCACGACTCAATTGGCCTGGGCGAAGATGGTCCTACGCACCAGGCTGTTGAGCAACTGGCCAGT
CTGCGCTTAACGCCAAATTTCAGCACCTGGCGACCGTGCGATCAGGTGGAAGCAGCGGTGGGCTGGAAGCTGGCGGTTGA
GCGCCACAACGGACCGACGGCACTGATCCTCTCCAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAG
AGATTGCTCGTGGTGGTTATGTGTTGAAAGACAGCGGCGGTAAGCCAGATATTATTTTGATTGCCACCGGTTCAGAGATG
GAAATCACCCTGCAAGCGGCAGAGAAATTAGCGGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATAT
TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTAGCGGTGGAAGCAGGCA
TCGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCG
GATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGC
CTGA

Domains

Predicted by InterProScan.

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(3-333)

Transketolase, pyrimidine binding domain (Transket_pyr)

(353-523)

Transketolase, C-terminal domain (Transketolase_C)

(539-654)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P33570

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.496

98.801

0.469