Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   MUK78_RS01440 Genome accession   NZ_CP095088
Coordinates   310640..311404 (-) Length   254 a.a.
NCBI ID   WP_001136236.1    Uniprot ID   A7ZT17
Organism   Escherichia coli isolate w264     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 305640..316404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUK78_RS01410 (MUK78_01410) - 306932..307213 (-) 282 WP_000084022.1 PTS sugar transporter subunit IIB -
  MUK78_RS01415 (MUK78_01415) - 307210..307683 (-) 474 WP_001161553.1 PTS sugar transporter subunit IIA -
  MUK78_RS01425 (MUK78_01425) - 308485..309231 (-) 747 WP_000839066.1 GntR family transcriptional regulator -
  MUK78_RS01430 (MUK78_01430) nikR 309430..309831 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  MUK78_RS01435 (MUK78_01435) nikE 309837..310643 (-) 807 WP_000173666.1 nickel import ATP-binding protein NikE -
  MUK78_RS01440 (MUK78_01440) amiE 310640..311404 (-) 765 WP_001136236.1 nickel import ATP-binding protein NikD Regulator
  MUK78_RS01445 (MUK78_01445) nikC 311404..312237 (-) 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
  MUK78_RS01450 (MUK78_01450) nikB 312234..313178 (-) 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
  MUK78_RS01455 (MUK78_01455) nikA 313178..314752 (-) 1575 WP_000953356.1 nickel ABC transporter substrate-binding protein -
  MUK78_RS01460 (MUK78_01460) acpT 314863..315450 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26833.42 Da        Isoelectric Point: 6.5992

>NTDB_id=582070 MUK78_RS01440 WP_001136236.1 310640..311404(-) (amiE) [Escherichia coli isolate w264]
MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAATLGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLHTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGKIVEQGDVETLFNAPKHTVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=582070 MUK78_RS01440 WP_001136236.1 310640..311404(-) (amiE) [Escherichia coli isolate w264]
ATGCCGCAACAGATTGAACTACGTAATATCGCGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGACGCTGGGCATTTTGC
CCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCT
GGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACCGACCTCGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAAGCGCCGGGAATGCTGCTGGTCACCCATGATATGGGCGTTGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCACAAGGTAAAATTGTCGAACAGGGCGATGTAGAAACGCTGTTTAACGCCCCCAAACATACGGTGACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZT17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

41.6

98.425

0.409
  amiE Streptococcus thermophilus LMD-9

41.6

98.425

0.409
  amiE Streptococcus salivarius strain HSISS4

40.4

98.425

0.398