Detailed information NTG0581977

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: MUK77_RS00825
• Genome accession: NZ_CP095086
• Coordinates: 175123..176106 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196477.1
• Uniprot ID: A0A0H2VBW7
• Organism: Escherichia coli isolate EC5654
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 170123..181106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MUK77_RS00810 (MUK77_00810)	dppA	171423..173030 (+)	1608	WP_001222871.1	dipeptide ABC transporter substrate-binding protein DppA	-
MUK77_RS00815 (MUK77_00815)	dppB	173181..174200 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
MUK77_RS00820 (MUK77_00820)	dppC	174210..175112 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
MUK77_RS00825 (MUK77_00825)	amiE	175123..176106 (+)	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	Regulator
MUK77_RS00830 (MUK77_00830)	dppF	176103..177107 (+)	1005	WP_000103583.1	dipeptide ABC transporter ATP-binding subunit DppF	-
MUK77_RS00835 (MUK77_00835)	yhjV	177137..178408 (-)	1272	WP_001467807.1	amino acid permease	-
MUK77_RS00840 (MUK77_00840)	ldrD	178884..178991 (+)	108	WP_000170745.1	type I toxin-antitoxin system toxin Ldr family protein	-
MUK77_RS00845 (MUK77_00845)	bcsG	179078..180757 (-)	1680	WP_000191567.1	cellulose biosynthesis protein BcsG	-
MUK77_RS00850 (MUK77_00850)	bcsF	180754..180945 (-)	192	WP_000988311.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35801.38 Da
• Isoelectric Point: 6.5813

>NTDB_id=581977 MUK77_RS00825 WP_001196477.1 175123..176106(+) (amiE) [Escherichia coli isolate EC5654]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=581977 MUK77_RS00825 WP_001196477.1 175123..176106(+) (amiE) [Escherichia coli isolate EC5654]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCTGGTAAATCGGTCAGTTCGCTGGCAATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTCAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCTGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATTATCGAACTGCTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTGATCACTCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTAGTAGAAACGGGCGATGCGCACGCT
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTATGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTACGCCA
CTGACAAATGCCGTGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• ABC transporter (ABC_tran) (24-181)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

3D structure

• AlphaFold DB: A0A0H2VBW7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404