Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6L62_RS16615 Genome accession   NZ_CP077411
Coordinates   3481547..3482980 (+) Length   477 a.a.
NCBI ID   WP_032659700.1    Uniprot ID   -
Organism   Enterobacter asburiae strain FDAARGOS 1432     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3476547..3487980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L62_RS16585 (I6L62_16585) - 3477114..3478091 (+) 978 WP_217071868.1 calcium/sodium antiporter -
  I6L62_RS16590 (I6L62_16590) kdsD 3478105..3479091 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  I6L62_RS16595 (I6L62_16595) kdsC 3479106..3479672 (+) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I6L62_RS16600 (I6L62_16600) lptC 3479669..3480244 (+) 576 WP_033146749.1 LPS export ABC transporter periplasmic protein LptC -
  I6L62_RS16605 (I6L62_16605) lptA 3480213..3480767 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  I6L62_RS16610 (I6L62_16610) lptB 3480774..3481499 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  I6L62_RS16615 (I6L62_16615) rpoN/rpoN1 3481547..3482980 (+) 1434 WP_032659700.1 RNA polymerase factor sigma-54 Machinery gene
  I6L62_RS16620 (I6L62_16620) hpf 3483003..3483290 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  I6L62_RS16625 (I6L62_16625) ptsN 3483373..3483864 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6L62_RS16630 (I6L62_16630) rapZ 3483910..3484764 (+) 855 WP_014885397.1 RNase adapter RapZ -
  I6L62_RS16635 (I6L62_16635) npr 3484761..3485033 (+) 273 WP_047058971.1 PTS phosphocarrier protein NPr -
  I6L62_RS16640 (I6L62_16640) mtgA 3485073..3485798 (-) 726 WP_217071869.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  I6L62_RS16645 (I6L62_16645) elbB 3485795..3486448 (-) 654 WP_217073232.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=581402 I6L62_RS16615 WP_032659700.1 3481547..3482980(+) (rpoN/rpoN1) [Enterobacter asburiae strain FDAARGOS 1432]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=581402 I6L62_RS16615 WP_032659700.1 3481547..3482980(+) (rpoN/rpoN1) [Enterobacter asburiae strain FDAARGOS 1432]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAGACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTTATCCCGGACGTGCTGGTGAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAAAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48