Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6L25_RS13180 Genome accession   NZ_CP077398
Coordinates   2816213..2817013 (-) Length   266 a.a.
NCBI ID   WP_004710659.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain FDAARGOS 1395     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2811213..2822013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L25_RS13160 (I6L25_13160) - 2812278..2812976 (-) 699 WP_002053293.1 YfgM family protein -
  I6L25_RS13165 (I6L25_13165) hisS 2813005..2814297 (-) 1293 WP_004710657.1 histidine--tRNA ligase -
  I6L25_RS13170 (I6L25_13170) ispG 2814294..2815409 (-) 1116 WP_004710658.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6L25_RS13175 (I6L25_13175) - 2815431..2816222 (-) 792 WP_002053491.1 helix-turn-helix domain-containing protein -
  I6L25_RS13180 (I6L25_13180) pilF 2816213..2817013 (-) 801 WP_004710659.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6L25_RS13185 (I6L25_13185) rlmN 2817029..2818261 (-) 1233 WP_002053401.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6L25_RS13190 (I6L25_13190) ndk 2818384..2818815 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  I6L25_RS13195 (I6L25_13195) iscX 2818929..2819129 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  I6L25_RS13200 (I6L25_13200) - 2819229..2819780 (-) 552 WP_004710660.1 phosphatase PAP2 family protein -
  I6L25_RS13205 (I6L25_13205) - 2819805..2821103 (-) 1299 WP_023188485.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30097.44 Da        Isoelectric Point: 9.2307

>NTDB_id=581175 I6L25_RS13180 WP_004710659.1 2816213..2817013(-) (pilF) [Acinetobacter nosocomialis strain FDAARGOS 1395]
MSTPKLKIMLCMGVAVAFLASGCQSSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=581175 I6L25_RS13180 WP_004710659.1 2816213..2817013(-) (pilF) [Acinetobacter nosocomialis strain FDAARGOS 1395]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCTGTTGCATTTCTGGCCAGTGGTTGTCAGTCTTCGCC
AACGCTTAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCCGCAGAATACATTCGTTCTGGTGACTTGG
ACTCTGCAAAGCGTTCCCTTGACCAAGCTTTAAGTGTCGATAGCCGTGATGCAACGGCAAATATGATGATGGGCATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCATCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCCATTGAGCAATTCCGTATTG
CTGGTGCAACATTAGGTTATGATCAACGCTATCAAGCCTTAGAAAATCTAGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGATTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATACGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

98.496

100

0.985
  pilF Acinetobacter baylyi ADP1

74.615

97.744

0.729