Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6L24_RS06325 Genome accession   NZ_CP077369
Coordinates   1358640..1359440 (+) Length   266 a.a.
NCBI ID   WP_005103248.1    Uniprot ID   N9QCA1
Organism   Acinetobacter lwoffii strain FDAARGOS 1394     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1353640..1364440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L24_RS06295 (I6L24_06295) - 1353933..1354343 (+) 411 WP_005103244.1 hypothetical protein -
  I6L24_RS06300 (I6L24_06300) - 1354585..1355865 (+) 1281 WP_005103246.1 glycosyltransferase family 4 protein -
  I6L24_RS06305 (I6L24_06305) - 1355886..1356437 (+) 552 WP_004278361.1 phosphatase PAP2 family protein -
  I6L24_RS06310 (I6L24_06310) iscX 1356525..1356725 (+) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  I6L24_RS06315 (I6L24_06315) ndk 1356836..1357267 (+) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  I6L24_RS06320 (I6L24_06320) rlmN 1357390..1358625 (+) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6L24_RS06325 (I6L24_06325) pilF 1358640..1359440 (+) 801 WP_005103248.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6L24_RS06330 (I6L24_06330) - 1359431..1360222 (+) 792 WP_004647463.1 helix-turn-helix domain-containing protein -
  I6L24_RS06335 (I6L24_06335) ispG 1360246..1361367 (+) 1122 WP_004278355.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6L24_RS06340 (I6L24_06340) hisS 1361371..1362666 (+) 1296 WP_005103249.1 histidine--tRNA ligase -
  I6L24_RS06345 (I6L24_06345) - 1362697..1363395 (+) 699 WP_005103250.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30119.24 Da        Isoelectric Point: 9.2878

>NTDB_id=580994 I6L24_RS06325 WP_005103248.1 1358640..1359440(+) (pilF) [Acinetobacter lwoffii strain FDAARGOS 1394]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=580994 I6L24_RS06325 WP_005103248.1 1358640..1359440(+) (pilF) [Acinetobacter lwoffii strain FDAARGOS 1394]
TTGAGAAATCTAACATCTAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATTTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
TTGCAGCAGGAAGGTAGCAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCACAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGTGCATTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QCA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

97.744

0.767
  pilF Acinetobacter baylyi ADP1

69.549

100

0.695