Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6L30_RS08190 Genome accession   NZ_CP077365
Coordinates   1742319..1743119 (-) Length   266 a.a.
NCBI ID   WP_004698762.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain FDAARGOS 1400     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1737319..1748119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L30_RS08170 (I6L30_08170) - 1738384..1739082 (-) 699 WP_107973576.1 YfgM family protein -
  I6L30_RS08175 (I6L30_08175) hisS 1739111..1740403 (-) 1293 WP_086496138.1 histidine--tRNA ligase -
  I6L30_RS08180 (I6L30_08180) ispG 1740400..1741515 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6L30_RS08185 (I6L30_08185) - 1741537..1742328 (-) 792 WP_004698761.1 helix-turn-helix domain-containing protein -
  I6L30_RS08190 (I6L30_08190) pilF 1742319..1743119 (-) 801 WP_004698762.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6L30_RS08195 (I6L30_08195) rlmN 1743135..1744367 (-) 1233 WP_004698763.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6L30_RS08200 (I6L30_08200) ndk 1744490..1744921 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  I6L30_RS08205 (I6L30_08205) iscX 1745035..1745235 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  I6L30_RS08210 (I6L30_08210) - 1745335..1745886 (-) 552 WP_019458079.1 phosphatase PAP2 family protein -
  I6L30_RS08215 (I6L30_08215) - 1745911..1747209 (-) 1299 WP_216985816.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30125.50 Da        Isoelectric Point: 9.2307

>NTDB_id=580917 I6L30_RS08190 WP_004698762.1 1742319..1743119(-) (pilF) [Acinetobacter seifertii strain FDAARGOS 1400]
MSTPKLKIMLCMGVAVAFLASGCQTSPTLKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSIESRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=580917 I6L30_RS08190 WP_004698762.1 1742319..1743119(-) (pilF) [Acinetobacter seifertii strain FDAARGOS 1400]
TTGAGTACTCCGAAGTTAAAGATCATGTTATGTATGGGGGTTGCAGTTGCATTTCTGGCAAGTGGTTGTCAGACGTCGCC
AACACTAAAAAAGGACCCGGAAAAAGCAGTTAAGGTGCGTACGCAGCTTGCTGCAGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAACGTTCCCTCGACCAAGCTCTAAGTATCGAGAGTCGTGACGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAACGTGCAATTTCTTCTGAGCCAGATAA
TGCTCAGGCGCGTAATAATTATGGTACGTATTTGTATCAAATGGAACGTTATAATGATGCTGTCGAGCAGTTCCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGTTACCAAGCCTTAGAAAATCTGGGGCGGATTTATTTAAAATTAGGTGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCGCTCCTTGCTAACCGTGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCTCGTGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAATCAA
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726