Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6L45_RS08275 Genome accession   NZ_CP077363
Coordinates   1743244..1744683 (+) Length   479 a.a.
NCBI ID   WP_216983929.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1415     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1738244..1749683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L45_RS08245 (I6L45_08245) mlaF 1738822..1739622 (-) 801 WP_043762636.1 phospholipid ABC transporter ATP-binding protein MlaF -
  I6L45_RS08250 (I6L45_08250) - 1739829..1740824 (+) 996 WP_043762637.1 KpsF/GutQ family sugar-phosphate isomerase -
  I6L45_RS08255 (I6L45_08255) kdsC 1740824..1741378 (+) 555 WP_216983927.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I6L45_RS08260 (I6L45_08260) lptC 1741375..1741935 (+) 561 WP_216983928.1 LPS export ABC transporter periplasmic protein LptC -
  I6L45_RS08265 (I6L45_08265) lptA 1741922..1742440 (+) 519 WP_043762680.1 lipopolysaccharide transport periplasmic protein LptA -
  I6L45_RS08270 (I6L45_08270) lptB 1742453..1743178 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  I6L45_RS08275 (I6L45_08275) rpoN/rpoN1 1743244..1744683 (+) 1440 WP_216983929.1 RNA polymerase factor sigma-54 Machinery gene
  I6L45_RS08280 (I6L45_08280) hpf 1744704..1744991 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  I6L45_RS08285 (I6L45_08285) ptsN 1744994..1745440 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6L45_RS08290 (I6L45_08290) rapZ 1745481..1746347 (+) 867 WP_216983930.1 RNase adapter RapZ -
  I6L45_RS08295 (I6L45_08295) npr 1746367..1746639 (+) 273 WP_010675868.1 PTS phosphocarrier protein NPr -
  I6L45_RS08300 (I6L45_08300) - 1746656..1748560 (-) 1905 WP_216983931.1 transcription antiterminator -
  I6L45_RS08305 (I6L45_08305) - 1748663..1749403 (-) 741 WP_216983932.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53803.74 Da        Isoelectric Point: 4.4290

>NTDB_id=580894 I6L45_RS08275 WP_216983929.1 1743244..1744683(+) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1415]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDAQETSEAEPHDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEESLYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTASLED
IQAAVSSEEVEVELDEIEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPELPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKEIDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=580894 I6L45_RS08275 WP_216983929.1 1743244..1744683(+) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1415]
ATGAAGCCGACATTACAGTTGCGCCTCGGTCAGTCACTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAGAACGATG
CGCAGGAAACCAGTGAGGCGGAGCCACACGATGCTAGTCAGCTCGACTCCAGCGATGCCATGGCACAGGACAAGATGCCG
GACGAGCTCCCTGTCGACACCACCTGGGACGAGGTCTATTCGGCCGGTACGGCCCAGAGTGCAGGCCCGATCCACGACGG
GGAGGAGAGCCTCTATCAGGGTGAAACCACCGAGAACCTGCAGGACTACCTGCTGTGGCAGATGCGTCTCACCCCCTTCA
GCGATGTGGACGCCGCCATCGCGCTTGCCATCATCGATGCCATCGATGAATCCGGCTATCTGACCGCTTCTCTCGAGGAC
ATTCAGGCTGCAGTCAGCAGCGAAGAGGTAGAAGTTGAACTGGATGAGATCGAAGCGGTACTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTTATCCAGCTCGGCCAGTATTCGCCCGAGTTGCCCT
GGCTCAACGAGGCCAGGGAGGTGATCCGCGAGCACATGGACTTGCTTGGCAATCGCGACTATCGCACTCTGGCACGTAAG
ACCCGGCTCAAGGAGATCGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCCAGGCCCGGCAACGGCATCAT
CCAGAGCGAATCCCAATACGTGATTCCGGATGTGTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTGGAGCTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAATGAGGCCTATGCGGCCATGGCACGCAATGCCCGCAGCAGCACGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCCAAATGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAGTATGGTGAAGAGGCGATGAAACCCATGGTGCTCAACGACATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATCTCGCGGGTCACGACCCAGAAGTACATGCACACGCCTCGCGGCATTTTTGAA
CTGAAGTTTTTTTTCTCCAGTCACGTCAATACGGAGGGAGGAGGAGAGTGCTCATCGACCGCTATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCGGAGAACCCCGCCAAGCCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.184

100

0.493