Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6L46_RS15290 Genome accession   NZ_CP077301
Coordinates   3387757..3389196 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas sp. FDAARGOS 1416     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3382757..3394196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L46_RS15260 (I6L46_15260) - 3383025..3383765 (+) 741 WP_042079669.1 KDGP aldolase family protein -
  I6L46_RS15265 (I6L46_15265) - 3383873..3385777 (+) 1905 WP_216976209.1 transcription antiterminator -
  I6L46_RS15270 (I6L46_15270) npr 3385795..3386067 (-) 273 WP_042024530.1 PTS phosphocarrier protein NPr -
  I6L46_RS15275 (I6L46_15275) rapZ 3386088..3386954 (-) 867 WP_124928328.1 RNase adapter RapZ -
  I6L46_RS15280 (I6L46_15280) ptsN 3387000..3387446 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6L46_RS15285 (I6L46_15285) hpf 3387449..3387736 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  I6L46_RS15290 (I6L46_15290) rpoN/rpoN1 3387757..3389196 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  I6L46_RS15295 (I6L46_15295) lptB 3389262..3389987 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  I6L46_RS15300 (I6L46_15300) lptA 3389991..3390518 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  I6L46_RS15305 (I6L46_15305) lptC 3390505..3391065 (-) 561 WP_120416082.1 LPS export ABC transporter periplasmic protein LptC -
  I6L46_RS15310 (I6L46_15310) kdsC 3391062..3391616 (-) 555 WP_101530526.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I6L46_RS15315 (I6L46_15315) - 3391616..3392611 (-) 996 WP_005336342.1 KpsF/GutQ family sugar-phosphate isomerase -
  I6L46_RS15320 (I6L46_15320) mlaF 3392819..3393619 (+) 801 WP_216976211.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=580660 I6L46_RS15290 WP_005336331.1 3387757..3389196(-) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1416]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=580660 I6L46_RS15290 WP_005336331.1 3387757..3389196(-) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1416]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATTCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGCTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCACAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGTAACGGCATCAT
CCAGAGCGACTCCCAGTATGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGAAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491