Detailed information NTG0580168

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6L29_RS11470
• Genome accession: NZ_CP077238
• Coordinates: 2429687..2430805 (+)
• Length: 372 a.a.
• NCBI ID: WP_002121759.1
• Uniprot ID: A0A022J839
• Organism: Acinetobacter pittii strain FDAARGOS 1399
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2424687..2435805

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6L29_RS11460 (I6L29_11460)	yggS	2427811..2428503 (-)	693	WP_005075313.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6L29_RS11465 (I6L29_11465)	pilT	2428630..2429667 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
I6L29_RS11470 (I6L29_11470)	pilU	2429687..2430805 (+)	1119	WP_002121759.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I6L29_RS11475 (I6L29_11475)	fur	2430875..2431312 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
I6L29_RS11480 (I6L29_11480)	omlA	2431424..2431822 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
I6L29_RS11485 (I6L29_11485)	-	2431887..2432201 (-)	315	WP_002121714.1	RnfH family protein	-
I6L29_RS11490 (I6L29_11490)	-	2432201..2433268 (-)	1068	WP_005075306.1	hypothetical protein	-
I6L29_RS11495 (I6L29_11495)	-	2433422..2433877 (-)	456	WP_002121698.1	bacteriohemerythrin	-
I6L29_RS11500 (I6L29_11500)	-	2434129..2434977 (-)	849	WP_002121748.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41945.18 Da
• Isoelectric Point: 6.6404

>NTDB_id=580168 I6L29_RS11470 WP_002121759.1 2429687..2430805(+) (pilU) [Acinetobacter pittii strain FDAARGOS 1399]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRTREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=580168 I6L29_RS11470 WP_002121759.1 2429687..2430805(+) (pilU) [Acinetobacter pittii strain FDAARGOS 1399]
ATGGATTTTAACGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGACAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAGACTCGTGAATGTAATTTTGCCATTATGAACCGCGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAACAGCGTGACATGCCCGGCATGGTACTACGTCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCCAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGCTGCATCATTACCCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATCCGTACGCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACGGGTCACTTGGTCTTAGCCACGATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
ACAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCAGCGATCGAGATTCTCATTAACTCACCACTAATTTCAGACT
ATATTCGCAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTCTATAAGTCAGGACAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTACG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A022J839

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	69.003	99.731	0.688
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363