Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6L34_RS08990 Genome accession   NZ_CP077237
Coordinates   1935934..1937373 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas sp. FDAARGOS 1404     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1930934..1942373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L34_RS08960 (I6L34_08960) - 1931202..1931942 (+) 741 WP_058058617.1 KDGP aldolase family protein -
  I6L34_RS08965 (I6L34_08965) - 1932050..1933954 (+) 1905 WP_058057522.1 transcription antiterminator -
  I6L34_RS08970 (I6L34_08970) npr 1933972..1934244 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  I6L34_RS08975 (I6L34_08975) rapZ 1934265..1935131 (-) 867 WP_005356665.1 RNase adapter RapZ -
  I6L34_RS08980 (I6L34_08980) ptsN 1935177..1935623 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6L34_RS08985 (I6L34_08985) hpf 1935626..1935913 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  I6L34_RS08990 (I6L34_08990) rpoN/rpoN1 1935934..1937373 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  I6L34_RS08995 (I6L34_08995) lptB 1937439..1938164 (-) 726 WP_058057521.1 LPS export ABC transporter ATP-binding protein -
  I6L34_RS09000 (I6L34_09000) lptA 1938168..1938695 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  I6L34_RS09005 (I6L34_09005) lptC 1938682..1939242 (-) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  I6L34_RS09010 (I6L34_09010) kdsC 1939239..1939793 (-) 555 WP_075115821.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I6L34_RS09015 (I6L34_09015) - 1939793..1940788 (-) 996 WP_058058615.1 KpsF/GutQ family sugar-phosphate isomerase -
  I6L34_RS09020 (I6L34_09020) mlaF 1940996..1941796 (+) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=580138 I6L34_RS08990 WP_005336331.1 1935934..1937373(-) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1404]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=580138 I6L34_RS08990 WP_005336331.1 1935934..1937373(-) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1404]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTACCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACAGCCCAAAGTGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGAGAGACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTTACCAGCGATGACGTAGAGGTGGAGCTCGATGAGGTCGAGGCGGTACTCAAGCGGGTTCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTCATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGTGACTCTCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCTGCCCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTTTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCCATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491