Detailed information    

experimental Experimentally validated

Overview

Name   epdK   Type   Machinery gene
Locus tag   MMJJ_RS05240 Genome accession   NZ_CP026606
Coordinates   968722..969780 (+) Length   352 a.a.
NCBI ID   WP_013998533.1    Uniprot ID   G0H0W7
Organism   Methanococcus maripaludis strain DSM 2067     
Function   DNA uptake   
DNA binding and uptake

Function

The ΔepdJKL mutant exhibited a transformation efficiency that was 2 orders of magnitude lower than that of the wild type, confirming that the observed phenotypes were not due to secondary peptidase targets of EppA.


Genomic Context

Location: 963722..974780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS05220 (MMJJ_10530) fni 964162..965229 (-) 1068 WP_104837970.1 type 2 isopentenyl-diphosphate Delta-isomerase -
  MMJJ_RS05225 (MMJJ_10540) - 965732..966013 (+) 282 WP_011169986.1 Gar1/Naf1 family protein -
  MMJJ_RS05230 (MMJJ_10550) - 966032..967051 (+) 1020 WP_104837971.1 transcription initiation factor IIB -
  MMJJ_RS05235 (MMJJ_10560) epdL 967061..968710 (+) 1650 WP_011169984.1 type II/IV secretion system ATPase subunit Machinery gene
  MMJJ_RS05240 (MMJJ_10570) epdK 968722..969780 (+) 1059 WP_013998533.1 type II secretion system F family protein Machinery gene
  MMJJ_RS05245 (MMJJ_10580) epdJ 969785..970681 (+) 897 WP_104837972.1 type II secretion system F family protein Machinery gene
  MMJJ_RS05250 (MMJJ_10590) - 970708..971295 (-) 588 WP_104837973.1 HD domain-containing protein -
  MMJJ_RS05255 (MMJJ_10600) tfe 971306..971863 (-) 558 WP_104837974.1 transcription factor E -
  MMJJ_RS05260 (MMJJ_10610) - 971867..972916 (-) 1050 WP_104837975.1 ATP-grasp domain-containing protein -
  MMJJ_RS05265 (MMJJ_10620) mptA 972942..973889 (-) 948 WP_104837976.1 GTP cyclohydrolase MptA -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39789.67 Da        Isoelectric Point: 9.7599

>NTDB_id=58 MMJJ_RS05240 WP_013998533.1 968722..969780(+) (epdK) [Methanococcus maripaludis strain DSM 2067]
MKRKTEKKQGTFDKLANTLKGVKTPKKRKISRVGRSEYLKKIFERKTEDIHKDEILEFYEPYIDETPEVSIDLDDLLFEK
KEFGALGGYSRSFSYWVTNTSFLPSKRDYQYAGIVDERVYFLKMMIAAITTVVLFIIYGVLTGDVFSGVSNGVLLAVIIV
VGSIFYPKLKLTLFRGEIKIQVLMSILHLISMLNSGASVQESLKNIANNPEYGITSFEFRSIIKDINQGGYNFVEALERA
KMRTKIHIMRQLYDQLILAANKGGTQLLLENLYNEIVRESMSKIDSSKFQISNLGNLIFGIGLIIPFSGMIQSALGAQQG
FDGIINAIDLVMGKIGLMSTIIFTIFIKMKIE

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=58 MMJJ_RS05240 WP_013998533.1 968722..969780(+) (epdK) [Methanococcus maripaludis strain DSM 2067]
ATGAAAAGAAAAACTGAAAAAAAACAGGGTACTTTTGATAAACTCGCCAATACATTAAAAGGAGTTAAAACTCCGAAAAA
AAGAAAAATATCTAGGGTTGGAAGATCGGAATATTTAAAAAAAATCTTCGAAAGAAAAACTGAAGATATACATAAAGATG
AGATTTTAGAGTTTTACGAACCATATATCGATGAAACTCCCGAAGTAAGTATTGACTTAGATGATTTACTTTTTGAAAAA
AAAGAATTTGGAGCACTTGGCGGATACTCCAGATCTTTTTCATACTGGGTTACAAACACCTCATTTTTACCTTCTAAGAG
GGATTACCAGTATGCAGGTATTGTAGATGAGCGAGTTTATTTCTTAAAGATGATGATTGCAGCAATCACCACGGTTGTTT
TATTCATAATTTACGGTGTTTTGACCGGCGATGTATTTTCTGGCGTTTCTAATGGGGTACTTTTAGCAGTTATTATAGTT
GTTGGTAGTATATTTTATCCAAAACTAAAATTAACATTGTTTAGAGGGGAAATTAAAATTCAGGTGTTAATGAGTATATT
ACACCTTATTTCAATGTTAAACTCTGGTGCTTCTGTTCAGGAATCCTTGAAAAATATTGCAAACAATCCAGAATATGGAA
TTACCTCTTTTGAATTTAGAAGTATTATAAAAGATATTAATCAGGGTGGGTATAACTTCGTAGAAGCTCTCGAAAGAGCT
AAAATGAGGACTAAAATACATATAATGAGACAGCTTTACGACCAGTTGATTCTTGCAGCAAATAAGGGTGGAACACAGCT
TTTACTTGAAAATTTGTATAATGAAATTGTAAGAGAATCTATGTCAAAAATAGACAGTTCAAAATTCCAAATATCAAATT
TGGGAAATCTTATATTTGGTATTGGTTTGATTATCCCATTTTCAGGAATGATACAGTCAGCATTGGGTGCTCAGCAAGGA
TTTGACGGAATTATCAATGCTATTGACCTCGTGATGGGCAAAATAGGGTTAATGTCAACAATTATATTTACCATCTTTAT
TAAAATGAAGATTGAGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(187-311)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G0H0W7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2020) Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea. Journal of Bacteriology 202(21):e00355-20. [PMID: 32817089]