Detailed information    

experimental Experimentally validated

Overview

Name   epdJ   Type   Machinery gene
Locus tag   MMJJ_RS05245 Genome accession   NZ_CP026606
Coordinates   969785..970681 (+) Length   298 a.a.
NCBI ID   WP_104837972.1    Uniprot ID   A0A2L1CC36
Organism   Methanococcus maripaludis strain DSM 2067     
Function   DNA uptake   
DNA binding and uptake

Function

The ΔepdJKL mutant exhibited a transformation efficiency that was 2 orders of magnitude lower than that of the wild type, confirming that the observed phenotypes were not due to secondary peptidase targets of EppA.


Genomic Context

Location: 964785..975681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMJJ_RS05225 (MMJJ_10540) - 965732..966013 (+) 282 WP_011169986.1 Gar1/Naf1 family protein -
  MMJJ_RS05230 (MMJJ_10550) - 966032..967051 (+) 1020 WP_104837971.1 transcription initiation factor IIB -
  MMJJ_RS05235 (MMJJ_10560) epdL 967061..968710 (+) 1650 WP_011169984.1 type II/IV secretion system ATPase subunit Machinery gene
  MMJJ_RS05240 (MMJJ_10570) epdK 968722..969780 (+) 1059 WP_013998533.1 type II secretion system F family protein Machinery gene
  MMJJ_RS05245 (MMJJ_10580) epdJ 969785..970681 (+) 897 WP_104837972.1 type II secretion system F family protein Machinery gene
  MMJJ_RS05250 (MMJJ_10590) - 970708..971295 (-) 588 WP_104837973.1 HD domain-containing protein -
  MMJJ_RS05255 (MMJJ_10600) tfe 971306..971863 (-) 558 WP_104837974.1 transcription factor E -
  MMJJ_RS05260 (MMJJ_10610) - 971867..972916 (-) 1050 WP_104837975.1 ATP-grasp domain-containing protein -
  MMJJ_RS05265 (MMJJ_10620) mptA 972942..973889 (-) 948 WP_104837976.1 GTP cyclohydrolase MptA -
  MMJJ_RS05270 (MMJJ_10630) - 973910..975136 (-) 1227 WP_011169977.1 AAA family ATPase -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 32768.42 Da        Isoelectric Point: 9.9536

>NTDB_id=57 MMJJ_RS05245 WP_104837972.1 969785..970681(+) (epdJ) [Methanococcus maripaludis strain DSM 2067]
MDAKKYLDHVYHVLIVRNIKILKKTGRKLDERVFIGILLVITILPILLKIFLGFTLKTTLILTFVYLGSVLSLPTIMYES
KMDKFDKNIPKALYVMVLSLDSGRSVVEAINEVIRSGIPEVDVVFSKIVTLMTERKLSFEDAMILVSNSLDSKIFRQVGR
LIIENRKYGGELADTLKKLAKTLDDLQNLKSQLLSVTANGLAVGLIILCGVIPATAGLIGGYLTVISQLAPTMPSVEASQ
ISKAIETIQMGSGLFGLFFAVPLFGLKVNRMIITCAVCMTFAIGTFYAVLRLTGLLFA

Nucleotide


Download         Length: 897 bp        

>NTDB_id=57 MMJJ_RS05245 WP_104837972.1 969785..970681(+) (epdJ) [Methanococcus maripaludis strain DSM 2067]
ATGGATGCCAAAAAATACCTAGATCATGTATATCATGTTTTAATTGTACGAAATATTAAGATATTAAAAAAAACAGGTAG
AAAACTTGATGAACGAGTTTTTATCGGAATATTATTGGTTATAACGATTTTACCAATATTGTTAAAAATATTTTTAGGTT
TCACCTTAAAAACTACCTTGATTTTAACTTTTGTATATTTAGGATCCGTTCTTTCACTCCCGACGATTATGTATGAATCT
AAAATGGATAAATTCGACAAAAATATACCTAAAGCACTATACGTCATGGTTTTATCACTTGATTCCGGACGTTCTGTTGT
TGAAGCAATTAATGAAGTTATAAGAAGTGGAATTCCTGAAGTGGACGTAGTTTTTTCAAAAATTGTTACATTAATGACTG
AAAGAAAATTGAGTTTTGAAGATGCAATGATACTTGTTTCAAATTCTCTTGATTCAAAGATATTTAGACAGGTTGGAAGG
TTAATCATTGAAAATAGAAAATATGGTGGAGAGCTTGCAGACACTCTGAAAAAACTTGCAAAAACACTTGACGACCTTCA
AAATTTAAAATCCCAACTTCTAAGTGTCACTGCAAACGGTCTTGCAGTGGGCCTTATAATTCTCTGTGGTGTGATTCCTG
CAACTGCTGGTTTGATTGGGGGTTATTTAACAGTAATATCTCAGTTAGCACCAACAATGCCGTCAGTTGAAGCTTCACAA
ATATCAAAAGCGATTGAAACGATACAGATGGGTTCTGGATTATTCGGGCTGTTTTTTGCAGTTCCATTATTTGGTTTAAA
AGTAAATAGAATGATAATTACGTGTGCGGTATGTATGACTTTTGCGATCGGCACATTTTATGCAGTATTGAGACTTACCG
GACTGTTGTTTGCATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(94-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2L1CC36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2020) Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea. Journal of Bacteriology 202(21):e00355-20. [PMID: 32817089]