Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6L66_RS00325 Genome accession   NZ_CP077223
Coordinates   60402..61835 (+) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain FDAARGOS 1436     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 55402..66835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L66_RS00295 (I6L66_00300) - 55969..56946 (+) 978 WP_020883528.1 calcium/sodium antiporter -
  I6L66_RS00300 (I6L66_00305) kdsD 56960..57946 (+) 987 WP_040018741.1 arabinose-5-phosphate isomerase KdsD -
  I6L66_RS00305 (I6L66_00310) kdsC 57961..58527 (+) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I6L66_RS00310 (I6L66_00315) lptC 58524..59099 (+) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  I6L66_RS00315 (I6L66_00320) lptA 59068..59622 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  I6L66_RS00320 (I6L66_00325) lptB 59629..60354 (+) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  I6L66_RS00325 (I6L66_00330) rpoN/rpoN1 60402..61835 (+) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  I6L66_RS00330 (I6L66_00335) hpf 61858..62145 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  I6L66_RS00335 (I6L66_00340) ptsN 62228..62719 (+) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6L66_RS00340 (I6L66_00345) rapZ 62765..63619 (+) 855 WP_014171820.1 RNase adapter RapZ -
  I6L66_RS00345 (I6L66_00350) npr 63616..63888 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  I6L66_RS00350 (I6L66_00355) mtgA 63927..64652 (-) 726 WP_014171821.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  I6L66_RS00355 (I6L66_00360) elbB 64649..65302 (-) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=579965 I6L66_RS00325 WP_014171817.1 60402..61835(+) (rpoN/rpoN1) [Enterobacter ludwigii strain FDAARGOS 1436]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=579965 I6L66_RS00325 WP_014171817.1 60402..61835(+) (rpoN/rpoN1) [Enterobacter ludwigii strain FDAARGOS 1436]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATAGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTTTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAACTTTCTCAGTTCAGTAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486