Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   I6L44_RS14770 Genome accession   NZ_CP077210
Coordinates   3287705..3289144 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas sp. FDAARGOS 1414     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3282705..3294144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L44_RS14740 (I6L44_14740) - 3283001..3284362 (+) 1362 WP_216952220.1 Do family serine endopeptidase -
  I6L44_RS14745 (I6L44_14745) degS 3284472..3285596 (+) 1125 WP_216952221.1 outer membrane-stress sensor serine endopeptidase DegS -
  I6L44_RS14750 (I6L44_14750) npr 3285743..3286015 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  I6L44_RS14755 (I6L44_14755) rapZ 3286036..3286902 (-) 867 WP_040068818.1 RNase adapter RapZ -
  I6L44_RS14760 (I6L44_14760) ptsN 3286948..3287394 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  I6L44_RS14765 (I6L44_14765) hpf 3287397..3287684 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  I6L44_RS14770 (I6L44_14770) rpoN/rpoN1 3287705..3289144 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  I6L44_RS14775 (I6L44_14775) lptB 3289210..3289935 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  I6L44_RS14780 (I6L44_14780) lptA 3289939..3290466 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  I6L44_RS14785 (I6L44_14785) lptC 3290453..3291013 (-) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  I6L44_RS14790 (I6L44_14790) kdsC 3291010..3291564 (-) 555 WP_047438734.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  I6L44_RS14795 (I6L44_14795) - 3291564..3292559 (-) 996 WP_005356672.1 KpsF/GutQ family sugar-phosphate isomerase -
  I6L44_RS14800 (I6L44_14800) mlaF 3292767..3293567 (+) 801 WP_005336343.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=579873 I6L44_RS14770 WP_005336331.1 3287705..3289144(-) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1414]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=579873 I6L44_RS14770 WP_005336331.1 3287705..3289144(-) (rpoN/rpoN1) [Aeromonas sp. FDAARGOS 1414]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GATGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACACCGTTCA
GCGATGTGGATGCTGCCATCGCGCTCGCCATTATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCAGCCGTCACCAGCGATGACGTAGAGGTGGAGCTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGTATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATTTGATCCAGCTGCTGGAGCCGCGCCCCGGCAATGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTTGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGCATCCGGGTCAACGAGACTTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATTCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491