Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   I6L28_RS00535 Genome accession   NZ_CP077205
Coordinates   109534..110334 (-) Length   266 a.a.
NCBI ID   WP_216946680.1    Uniprot ID   -
Organism   Acinetobacter pittii strain FDAARGOS 1398     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 104534..115334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L28_RS00515 (I6L28_00515) - 105596..106294 (-) 699 WP_216946679.1 YfgM family protein -
  I6L28_RS00520 (I6L28_00520) hisS 106323..107615 (-) 1293 WP_062849551.1 histidine--tRNA ligase -
  I6L28_RS00525 (I6L28_00525) ispG 107612..108727 (-) 1116 WP_081175305.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  I6L28_RS00530 (I6L28_00530) - 108749..109543 (-) 795 WP_200043290.1 helix-turn-helix domain-containing protein -
  I6L28_RS00535 (I6L28_00535) pilF 109534..110334 (-) 801 WP_216946680.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  I6L28_RS00540 (I6L28_00540) rlmN 110349..111584 (-) 1236 WP_216946681.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  I6L28_RS00545 (I6L28_00545) ndk 111708..112139 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  I6L28_RS00550 (I6L28_00550) iscX 112253..112453 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  I6L28_RS00555 (I6L28_00555) - 112553..113104 (-) 552 WP_016144298.1 phosphatase PAP2 family protein -
  I6L28_RS00560 (I6L28_00560) - 113129..114415 (-) 1287 WP_079748238.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30086.41 Da        Isoelectric Point: 9.2541

>NTDB_id=579759 I6L28_RS00535 WP_216946680.1 109534..110334(-) (pilF) [Acinetobacter pittii strain FDAARGOS 1398]
MIIPKLKTVLCMGIALAFLASGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKTNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMGRYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
VNAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQALVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=579759 I6L28_RS00535 WP_216946680.1 109534..110334(-) (pilF) [Acinetobacter pittii strain FDAARGOS 1398]
TTGATTATTCCAAAGTTAAAAACGGTCTTATGTATGGGGATTGCACTTGCATTTCTGGCAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCGGAAAAAGCAGTAAAAGTGCGTACACAACTTGCTGCTGAACATATTCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACGGCGAATATGATGATGGGGATTTTG
CTACAGCAAGAAGGTAGCAAAACCAACTTAGAAAAAGCAGAACATTATTTTAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATACCAAATGGGGCGTTATAATGATGCGATCGAGCAATTTCGTATTG
CTGGTGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GTGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGTGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGCGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(89-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

92.857

100

0.929
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737