Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6L32_RS04220 Genome accession   NZ_CP077201
Coordinates   883647..884756 (+) Length   369 a.a.
NCBI ID   WP_216944735.1    Uniprot ID   -
Organism   Aeromonas sp. FDAARGOS 1402     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 878647..889756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L32_RS04185 (I6L32_04185) - 878682..879512 (+) 831 WP_216944733.1 CPBP family intramembrane glutamic endopeptidase -
  I6L32_RS04190 (I6L32_04190) - 879588..880007 (-) 420 WP_029313762.1 DUF4426 domain-containing protein -
  I6L32_RS04195 (I6L32_04195) yggU 880026..880325 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  I6L32_RS04200 (I6L32_04200) - 880325..880876 (-) 552 WP_042014922.1 YggT family protein -
  I6L32_RS04205 (I6L32_04205) proC 880900..881724 (-) 825 WP_216944734.1 pyrroline-5-carboxylate reductase -
  I6L32_RS04210 (I6L32_04210) - 881848..882549 (-) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L32_RS04215 (I6L32_04215) pilT 882589..883623 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  I6L32_RS04220 (I6L32_04220) pilU 883647..884756 (+) 1110 WP_216944735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6L32_RS04225 (I6L32_04225) yaaA 884789..885562 (+) 774 WP_216944736.1 peroxide stress protein YaaA -
  I6L32_RS04230 (I6L32_04230) srmB 885750..886970 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  I6L32_RS04235 (I6L32_04235) - 887098..887808 (+) 711 WP_202040292.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  I6L32_RS04240 (I6L32_04240) brnQ 888044..889306 (+) 1263 WP_254203880.1 branched-chain amino acid transport system II carrier protein -
  I6L32_RS04245 (I6L32_04245) - 889396..889623 (-) 228 WP_039039956.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40887.01 Da        Isoelectric Point: 6.3152

>NTDB_id=579734 I6L32_RS04220 WP_216944735.1 883647..884756(+) (pilU) [Aeromonas sp. FDAARGOS 1402]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=579734 I6L32_RS04220 WP_216944735.1 883647..884756(+) (pilU) [Aeromonas sp. FDAARGOS 1402]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACGGCGCTGGCGCTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTACATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379