Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6L32_RS04215 Genome accession   NZ_CP077201
Coordinates   882589..883623 (+) Length   344 a.a.
NCBI ID   WP_010673050.1    Uniprot ID   A0A081LS93
Organism   Aeromonas sp. FDAARGOS 1402     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877589..888623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6L32_RS04175 (I6L32_04175) - 877796..878170 (+) 375 WP_039039964.1 YacL family protein -
  I6L32_RS04180 (I6L32_04180) - 878220..878510 (+) 291 WP_216944732.1 hypothetical protein -
  I6L32_RS04185 (I6L32_04185) - 878682..879512 (+) 831 WP_216944733.1 CPBP family intramembrane glutamic endopeptidase -
  I6L32_RS04190 (I6L32_04190) - 879588..880007 (-) 420 WP_029313762.1 DUF4426 domain-containing protein -
  I6L32_RS04195 (I6L32_04195) yggU 880026..880325 (-) 300 WP_010673054.1 DUF167 family protein YggU -
  I6L32_RS04200 (I6L32_04200) - 880325..880876 (-) 552 WP_042014922.1 YggT family protein -
  I6L32_RS04205 (I6L32_04205) proC 880900..881724 (-) 825 WP_216944734.1 pyrroline-5-carboxylate reductase -
  I6L32_RS04210 (I6L32_04210) - 881848..882549 (-) 702 WP_042014924.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6L32_RS04215 (I6L32_04215) pilT 882589..883623 (+) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  I6L32_RS04220 (I6L32_04220) pilU 883647..884756 (+) 1110 WP_216944735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6L32_RS04225 (I6L32_04225) yaaA 884789..885562 (+) 774 WP_216944736.1 peroxide stress protein YaaA -
  I6L32_RS04230 (I6L32_04230) srmB 885750..886970 (-) 1221 WP_041210934.1 ATP-dependent RNA helicase SrmB -
  I6L32_RS04235 (I6L32_04235) - 887098..887808 (+) 711 WP_202040292.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.90 Da        Isoelectric Point: 6.7436

>NTDB_id=579733 I6L32_RS04215 WP_010673050.1 882589..883623(+) (pilT) [Aeromonas sp. FDAARGOS 1402]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALDHREVHALIYDIMNDHQRKELEENFEVDFSFEVPGMAR
FRVNAFQQARGSGAVFRTIPSTVLTLDDLGAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVDYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMLGIPAVRNLIREDKVAQLYSVIQTGMTHGMQTMDQSL
KQLVNRGVVASLDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=579733 I6L32_RS04215 WP_010673050.1 882589..883623(+) (pilT) [Aeromonas sp. FDAARGOS 1402]
ATGGATATCACAGAGTTATTGGCATTCAGTGTAAAGCATAAGGCCTCGGATCTACACCTCTCGGCCGGGGTCCCCCCGAT
GATCAGGGTTGATGGGGAGGTTCGCAAGATCAATTTGCCCGCCCTGGATCACCGGGAAGTCCATGCTCTCATTTACGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTGGACTTCTCCTTCGAAGTACCCGGCATGGCACGC
TTTCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCCGTGTTTCGTACCATCCCGAGCACCGTTCTGACCCTGGA
CGATCTGGGGGCGCCGGAGATATTTCGCAAGATAGCGGAGTACCCTCGCGGCCTGGTGCTGGTGACTGGGCCCACCGGCT
CGGGCAAATCCACCACCCTGGCCGCCATGGTCGATTACATCAATGACAACTTCCACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACGAGAACAAGCGCTGCCTGGTGAACCAGCGGGAAGTACACCGCGACACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCTTGCGGGAAGACCCGGATATCATCCTGGTAGGTGAAATGCGGGACCTCGAAACCATCCGTCTCG
CCATGACCGCCGCTGAAACCGGTCACTTGGTGTTTGGCACCCTGCACACCTCCTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCTCGGAGAAGGACATGGTGCGATCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGTATCGGTGGCGGCCGGGTGGCGGCCCACGAGATCATGCTGGGGATCCCGGCGGTGCGAAACCTCATCC
GGGAAGACAAGGTGGCCCAGCTCTATTCCGTCATCCAGACCGGGATGACCCACGGCATGCAGACCATGGACCAGAGCCTC
AAGCAGCTGGTCAACCGGGGGGTCGTCGCCTCCCTCGATGCCAAGGCCAAGGCGGTCGATCCCAATACCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081LS93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Pseudomonas stutzeri DSM 10701

78.299

99.128

0.776
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Pseudomonas aeruginosa PAK

76.833

99.128

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

71.471

98.837

0.706
  pilT Legionella pneumophila strain ERS1305867

71.471

98.837

0.706
  pilT Neisseria meningitidis 8013

69.027

98.547

0.68
  pilT Neisseria gonorrhoeae MS11

68.732

98.547

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.924

99.419

0.526
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407