Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SPN994039_RS09380 Genome accession   NC_021005
Coordinates   1743368..1744192 (-) Length   274 a.a.
NCBI ID   WP_001036786.1    Uniprot ID   A0A0B7LDF1
Organism   Streptococcus pneumoniae SPN994039     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1738368..1749192
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN994039_RS09375 (SPN994039_17160) - 1741746..1743077 (-) 1332 WP_000390039.1 hemolysin family protein -
  SPN994039_RS09380 (SPN994039_17170) endA 1743368..1744192 (-) 825 WP_001036786.1 DNA-entry nuclease EndA Machinery gene
  SPN994039_RS09385 (SPN994039_17180) - 1744231..1744419 (-) 189 WP_001036519.1 DNA-directed RNA polymerase subunit beta -
  SPN994039_RS09390 (SPN994039_17190) murA 1744412..1745695 (-) 1284 WP_000358034.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SPN994039_RS11895 (SPN994039_17200) - 1745719..1745949 (-) 231 WP_001809307.1 DUF1146 family protein -
  SPN994039_RS09395 (SPN994039_17210) sepM 1746007..1747044 (-) 1038 WP_000727274.1 SepM family pheromone-processing serine protease Regulator
  SPN994039_RS09400 (SPN994039_17220) coaD 1747028..1747516 (-) 489 WP_001280736.1 pantetheine-phosphate adenylyltransferase -
  SPN994039_RS09405 (SPN994039_17230) rsmD 1747506..1748045 (-) 540 WP_000706951.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SPN994039_RS09410 (SPN994039_17240) asnA 1748110..1749102 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29921.59 Da        Isoelectric Point: 10.1197

>NTDB_id=57963 SPN994039_RS09380 WP_001036786.1 1743368..1744192(-) (endA) [Streptococcus pneumoniae SPN994039]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=57963 SPN994039_RS09380 WP_001036786.1 1743368..1744192(-) (endA) [Streptococcus pneumoniae SPN994039]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7LDF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.635

100

0.996
  endA Streptococcus pneumoniae D39

99.635

100

0.996
  endA Streptococcus pneumoniae R6

99.635

100

0.996
  endA Streptococcus pneumoniae TIGR4

99.635

100

0.996

Multiple sequence alignment