Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HU773_RS23860 Genome accession   NZ_CP077085
Coordinates   5346152..5347378 (+) Length   408 a.a.
NCBI ID   WP_186625304.1    Uniprot ID   -
Organism   Pseudomonas shahriarae strain SWRI52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5341152..5352378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU773_RS23835 (HU773_023835) nadC 5342584..5343432 (+) 849 WP_186625302.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HU773_RS23840 (HU773_023840) - 5343499..5343804 (-) 306 WP_057444407.1 DUF6388 family protein -
  HU773_RS23850 (HU773_023850) - 5344054..5345460 (-) 1407 WP_186625303.1 O-antigen ligase family protein -
  HU773_RS23855 (HU773_023855) - 5345539..5345949 (-) 411 WP_186625305.1 pilin -
  HU773_RS23860 (HU773_023860) pilC 5346152..5347378 (+) 1227 WP_186625304.1 type II secretion system F family protein Machinery gene
  HU773_RS23865 (HU773_023865) pilD 5347387..5348253 (+) 867 WP_057958433.1 prepilin peptidase Machinery gene
  HU773_RS23870 (HU773_023870) coaE 5348250..5348873 (+) 624 WP_057958402.1 dephospho-CoA kinase -
  HU773_RS23875 (HU773_023875) yacG 5348870..5349073 (+) 204 WP_032861907.1 DNA gyrase inhibitor YacG -
  HU773_RS23880 (HU773_023880) - 5349070..5349285 (-) 216 WP_057441062.1 hypothetical protein -
  HU773_RS23885 (HU773_023885) - 5349349..5350038 (-) 690 WP_057441064.1 energy-coupling factor ABC transporter permease -
  HU773_RS23890 (HU773_023890) - 5350035..5350502 (-) 468 WP_057441067.1 hypothetical protein -
  HU773_RS23895 (HU773_023895) - 5350605..5351234 (+) 630 WP_057441069.1 DUF1780 domain-containing protein -
  HU773_RS23900 (HU773_023900) - 5351274..5351447 (+) 174 WP_057441071.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44275.03 Da        Isoelectric Point: 10.2214

>NTDB_id=578945 HU773_RS23860 WP_186625304.1 5346152..5347378(+) (pilC) [Pseudomonas shahriarae strain SWRI52]
MNDVTVNPAPTATYAWEGTLRNGRKVSGETRGHSPAVIKAQLRRQGINPVRVQMRSSLLSGLRNPITPADVALFTRQLAT
LLKAGIPLLQAFDMIGEGFENRAMRTMVQGLKQEIAAGNSLTGALLKQPQCFNALYCSLIAAGEQAGTLETLLERVATHL
EKSQRLKTRIKKAMTYPLVVLLVATLVSSILLIHVVPQFESLFAGIDTPLPRFTLLVIVLSEFMQSAWWMLLLAVAATTW
GIRRSYRRHSGFRLWLDAGLLKVPLAGTLLKKTAVARYARTLATTFAAGVPLAQALDSVAGAAGNERFKQAVTLMRQDVA
IGMQLNRTMTASGLFPGMAIQMTAIGEESGALDSMLEKVADHYETDVDNLVDNLTSLMEPLIMVVLGSIVGALVIAMYLP
IFQLGTAF

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=578945 HU773_RS23860 WP_186625304.1 5346152..5347378(+) (pilC) [Pseudomonas shahriarae strain SWRI52]
ATGAATGATGTCACCGTAAACCCGGCCCCCACCGCCACCTACGCCTGGGAAGGCACCCTGCGCAATGGCCGCAAGGTGTC
CGGCGAAACCCGCGGGCATAGCCCGGCCGTGATCAAGGCCCAATTGCGCCGACAAGGGATCAATCCGGTTCGGGTCCAGA
TGCGCTCCAGCCTCCTGTCTGGCCTGCGCAACCCCATCACCCCAGCCGATGTTGCGCTGTTCACCCGCCAACTGGCGACC
CTGCTGAAGGCGGGAATCCCCCTGCTGCAAGCCTTCGACATGATCGGCGAAGGCTTTGAAAACCGGGCCATGCGCACAAT
GGTCCAGGGATTGAAGCAGGAGATTGCCGCCGGCAACAGCCTGACCGGCGCGTTATTGAAACAACCGCAGTGCTTCAACG
CCCTGTATTGCAGCCTGATCGCGGCCGGCGAACAGGCCGGCACCCTGGAAACCCTGCTGGAGCGGGTCGCCACCCACCTG
GAAAAAAGCCAACGCCTCAAGACCCGGATCAAGAAAGCCATGACCTATCCACTGGTGGTCCTGCTCGTGGCCACCCTGGT
CAGCAGTATCTTGCTGATCCACGTCGTGCCCCAGTTCGAAAGCCTGTTCGCCGGCATCGACACGCCGCTGCCGCGCTTTA
CCCTGCTGGTTATTGTGCTGTCCGAATTCATGCAAAGCGCGTGGTGGATGCTGTTGCTGGCCGTGGCGGCGACTACTTGG
GGCATACGTCGCAGCTATCGTCGACACAGTGGTTTTCGTCTGTGGCTGGACGCTGGTTTGTTGAAAGTGCCATTGGCAGG
CACACTGCTGAAAAAAACCGCTGTCGCCCGCTACGCTCGCACCCTCGCCACCACCTTTGCCGCCGGCGTACCGCTGGCAC
AGGCGTTGGATTCAGTGGCGGGAGCCGCCGGCAACGAACGCTTCAAACAAGCAGTCACGCTTATGCGCCAGGACGTAGCA
ATAGGAATGCAATTGAATCGAACTATGACCGCCAGCGGCCTGTTCCCTGGCATGGCGATCCAGATGACAGCCATCGGCGA
AGAATCGGGCGCACTGGACAGCATGCTGGAGAAGGTCGCCGACCATTATGAAACGGACGTCGACAACCTGGTGGACAACC
TGACCAGCCTGATGGAACCGCTGATCATGGTGGTGCTCGGCAGCATTGTCGGGGCGCTGGTGATCGCCATGTACCTGCCG
ATCTTCCAGCTCGGCACGGCGTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

97.059

0.556
  pilC Acinetobacter baylyi ADP1

52.658

96.814

0.51
  pilC Acinetobacter baumannii D1279779

51.515

97.059

0.5
  pilC Legionella pneumophila strain ERS1305867

47.395

98.775

0.468
  pilC Vibrio cholerae strain A1552

40.152

97.059

0.39
  pilG Neisseria gonorrhoeae MS11

39.594

96.569

0.382
  pilG Neisseria meningitidis 44/76-A

38.832

96.569

0.375
  pilC Vibrio campbellii strain DS40M4

37.913

96.324

0.365