Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   SPN032672_RS06060 Genome accession   NC_021003
Coordinates   1131149..1131973 (+) Length   274 a.a.
NCBI ID   WP_001036790.1    Uniprot ID   A0A0B7KWT3
Organism   Streptococcus pneumoniae SPN032672     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1126149..1136973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPN032672_RS06025 asnA 1126239..1127231 (+) 993 WP_000747993.1 aspartate--ammonia ligase -
  SPN032672_RS06030 rsmD 1127296..1127835 (+) 540 WP_000706964.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SPN032672_RS06035 coaD 1127825..1128313 (+) 489 WP_001280723.1 pantetheine-phosphate adenylyltransferase -
  SPN032672_RS06040 sepM 1128297..1129334 (+) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  SPN032672_RS06045 - 1129392..1129622 (+) 231 WP_000250391.1 DUF1146 family protein -
  SPN032672_RS06050 murA 1129646..1130929 (+) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  SPN032672_RS06055 - 1130922..1131110 (+) 189 WP_001818955.1 DNA-directed RNA polymerase subunit beta -
  SPN032672_RS06060 endA 1131149..1131973 (+) 825 WP_001036790.1 DNA-entry nuclease EndA Machinery gene
  SPN032672_RS06065 - 1132264..1133595 (+) 1332 WP_000390043.1 hemolysin family protein -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29949.69 Da        Isoelectric Point: 10.1700

>NTDB_id=57883 SPN032672_RS06060 WP_001036790.1 1131149..1131973(+) (endA) [Streptococcus pneumoniae SPN032672]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=57883 SPN032672_RS06060 WP_001036790.1 1131149..1131973(+) (endA) [Streptococcus pneumoniae SPN032672]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGACAGCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGTAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7KWT3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.175

100

0.982
  endA Streptococcus pneumoniae D39

98.175

100

0.982
  endA Streptococcus pneumoniae R6

98.175

100

0.982
  endA Streptococcus pneumoniae TIGR4

98.175

100

0.982

Multiple sequence alignment