Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KSS94_RS25340 Genome accession   NZ_CP077076
Coordinates   5752841..5754049 (-) Length   402 a.a.
NCBI ID   WP_217843668.1    Uniprot ID   -
Organism   Pseudomonas fakonensis strain COW40     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5747841..5759049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSS94_RS25325 (KSS94_25325) - 5749495..5751105 (-) 1611 WP_217840757.1 AAA family ATPase -
  KSS94_RS25330 (KSS94_25330) aroB 5751121..5752218 (-) 1098 WP_217840758.1 3-dehydroquinate synthase -
  KSS94_RS25335 (KSS94_25335) aroK 5752318..5752836 (-) 519 WP_217840759.1 shikimate kinase AroK -
  KSS94_RS25340 (KSS94_25340) pilQ 5752841..5754049 (-) 1209 WP_217843668.1 type IV pilus secretin PilQ Machinery gene
  KSS94_RS25345 (KSS94_25345) - 5754015..5755037 (-) 1023 WP_217840760.1 pilus assembly protein PilP -
  KSS94_RS25350 (KSS94_25350) - 5755034..5755570 (-) 537 WP_217840761.1 PilN domain-containing protein -
  KSS94_RS25355 (KSS94_25355) pilM 5755570..5756490 (-) 921 WP_225935820.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43812.29 Da        Isoelectric Point: 5.0201

>NTDB_id=578716 KSS94_RS25340 WP_217843668.1 5752841..5754049(-) (pilQ) [Pseudomonas fakonensis strain COW40]
MSLVQASPQKGEPMSLNFQDVEVRSVLQVMADFAGINLVASDTVQGRITLRLEAVPWEQALDLVLRSKGLGRRMEGSVMV
VAPLAELAGQGEELEQMPLQRELLAVHHANAAELAELFRTSLAEEGAFAGRGSVSVDARTNTLVAVQPPSRMAELRELVT
RLDVPVRQVMIEARIVEAGVDYEKVLGVRWGRQLYGENARLGHELFVDLGVERATSGVGVGVGLLRSDILLDLELSAMEK
SGHGEIISQPKVVTADKETARIIKGTEVPYQETTKSGATSISFREASLSLEVTPQITPDGKVVMAVRVTKDEPDFVNALN
NVPPIRKNEVNAKVQVADGETIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMNDQAIAV
SR

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=578716 KSS94_RS25340 WP_217843668.1 5752841..5754049(-) (pilQ) [Pseudomonas fakonensis strain COW40]
ATGAGCCTCGTGCAGGCGTCGCCCCAGAAGGGTGAGCCGATGTCGCTGAACTTTCAGGATGTCGAGGTTCGTTCGGTGCT
GCAGGTGATGGCCGATTTTGCCGGCATCAACCTGGTGGCCAGCGATACGGTGCAGGGCCGCATCACCTTGCGCCTGGAAG
CGGTGCCCTGGGAGCAGGCGCTTGACCTGGTGCTGCGCAGCAAGGGGCTTGGCCGGCGCATGGAGGGCTCGGTAATGGTG
GTGGCGCCGCTCGCCGAGCTTGCCGGGCAGGGCGAGGAACTGGAGCAGATGCCATTGCAGCGGGAGCTGTTGGCCGTCCA
TCACGCCAATGCTGCCGAGTTGGCCGAGCTTTTTCGGACCAGCCTGGCCGAGGAAGGTGCTTTCGCCGGGCGGGGCAGCG
TCAGCGTGGATGCGCGGACCAACACATTGGTGGCGGTGCAGCCGCCGAGCCGGATGGCCGAGCTGCGCGAGCTTGTGACG
AGGCTGGACGTGCCCGTTCGTCAGGTCATGATCGAGGCGCGTATCGTCGAGGCGGGGGTGGATTACGAAAAAGTCCTCGG
GGTGCGCTGGGGGCGCCAGTTGTATGGGGAAAATGCCCGCCTGGGCCATGAACTGTTCGTCGACCTGGGGGTGGAGCGGG
CCACCTCGGGTGTCGGTGTCGGTGTCGGGCTGCTGCGCAGCGATATCCTGCTGGACCTGGAGCTGAGTGCCATGGAGAAA
AGCGGCCATGGCGAGATCATCTCGCAGCCCAAGGTGGTCACCGCCGACAAGGAGACGGCACGAATCATCAAGGGCACCGA
GGTGCCGTACCAGGAAACCACCAAAAGCGGCGCCACCTCGATTTCCTTTCGCGAAGCCTCGCTGTCGCTGGAGGTGACGC
CGCAGATCACCCCCGACGGCAAGGTGGTCATGGCGGTGCGGGTGACCAAGGATGAGCCTGACTTCGTCAATGCCCTCAAC
AACGTCCCGCCGATCCGCAAGAACGAGGTCAATGCCAAGGTGCAGGTGGCGGATGGCGAAACCATTGTGATTGGTGGCGT
GTACTCGACGTCGCAGAACAAAGTGGTGGATAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGAC
GCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAATGACCAGGCGATTGCTGTG
AGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(237-393)

Bacterial type II/III secretion system short domain (Secretin_N)

(104-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

57.569

100

0.624
  comQ Acinetobacter baylyi ADP1

43.243

100

0.478
  pilQ Acinetobacter baumannii strain A118

40.558

100

0.47
  pilQ Acinetobacter baumannii D1279779

40.558

100

0.47
  pilQ Legionella pneumophila strain Lp02

43.029

100

0.445
  pilQ Legionella pneumophila strain ERS1305867

43.029

100

0.445
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.758

100

0.428
  pilQ Vibrio cholerae strain A1552

40.758

100

0.428
  pilQ Vibrio campbellii strain DS40M4

39.059

100

0.413
  pilQ Neisseria meningitidis M1080

35.476

100

0.371
  pilQ Neisseria gonorrhoeae strain FA1090

35.252

100

0.366
  pilQ Neisseria meningitidis MC58

35

100

0.366