Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KRH48_RS13240 Genome accession   NZ_CP076839
Coordinates   2736235..2737209 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 55-100-016     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731235..2742209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KRH48_RS13205 (KRH48_13205) rpmG 2731695..2731844 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  KRH48_RS13210 (KRH48_13210) - 2732053..2732592 (+) 540 WP_000164554.1 5-formyltetrahydrofolate cyclo-ligase -
  KRH48_RS13215 (KRH48_13215) - 2732604..2734067 (+) 1464 WP_001017856.1 rhomboid family intramembrane serine protease -
  KRH48_RS13220 (KRH48_13220) - 2734048..2734251 (+) 204 WP_000087559.1 YqgQ family protein -
  KRH48_RS13225 (KRH48_13225) - 2734248..2735234 (+) 987 WP_000161315.1 ROK family glucokinase -
  KRH48_RS13230 (KRH48_13230) - 2735234..2735563 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KRH48_RS13235 (KRH48_13235) - 2735560..2736183 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KRH48_RS13240 (KRH48_13240) comGA 2736235..2737209 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KRH48_RS13245 (KRH48_13245) comGB 2737181..2738251 (+) 1071 WP_000776425.1 competence type IV pilus assembly protein ComGB Machinery gene
  KRH48_RS13250 (KRH48_13250) comGC 2738265..2738576 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KRH48_RS13255 (KRH48_13255) comGD 2738554..2739000 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  KRH48_RS13260 (KRH48_13260) comGE 2738987..2739286 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KRH48_RS13265 (KRH48_13265) comGF 2739204..2739701 (+) 498 WP_001825340.1 competence type IV pilus minor pilin ComGF Machinery gene
  KRH48_RS13270 (KRH48_13270) - 2739798..2739944 (+) 147 WP_001789879.1 hypothetical protein -
  KRH48_RS13275 (KRH48_13275) - 2739934..2740458 (+) 525 WP_001015120.1 shikimate kinase -
  KRH48_RS13280 (KRH48_13280) gcvT 2740617..2741708 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=578467 KRH48_RS13240 WP_000697220.1 2736235..2737209(+) (comGA) [Staphylococcus aureus strain 55-100-016]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=578467 KRH48_RS13240 WP_000697220.1 2736235..2737209(+) (comGA) [Staphylococcus aureus strain 55-100-016]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTACGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1