Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ECBG_RS09900 Genome accession   NC_020995
Coordinates   2157320..2159137 (-) Length   605 a.a.
NCBI ID   WP_015509978.1    Uniprot ID   M9T6H0
Organism   Enterococcus casseliflavus EC20     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2152320..2164137
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECBG_RS09875 (ECBG_02006) - 2153277..2154176 (-) 900 WP_015509975.1 RluA family pseudouridine synthase -
  ECBG_RS09880 (ECBG_02005) - 2154181..2154978 (-) 798 WP_026008090.1 NAD kinase -
  ECBG_RS09885 (ECBG_02004) - 2154984..2155652 (-) 669 WP_005229708.1 GTP pyrophosphokinase family protein -
  ECBG_RS09890 (ECBG_02003) - 2155849..2156424 (+) 576 WP_015509976.1 CYTH domain-containing protein -
  ECBG_RS09895 (ECBG_02002) - 2156533..2157189 (+) 657 WP_015509977.1 ClpXP adapter SpxH family protein -
  ECBG_RS09900 (ECBG_02000) pepF 2157320..2159137 (-) 1818 WP_015509978.1 oligoendopeptidase F Regulator
  ECBG_RS09905 (ECBG_01999) - 2159206..2160195 (-) 990 WP_015509979.1 competence protein CoiA family protein -
  ECBG_RS09910 (ECBG_01998) - 2160296..2160946 (-) 651 WP_005229698.1 adaptor protein MecA -
  ECBG_RS09915 (ECBG_01997) spxA 2161099..2161497 (-) 399 WP_003127030.1 transcriptional regulator SpxA -
  ECBG_RS09920 (ECBG_01996) - 2162207..2163943 (-) 1737 WP_015509980.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 605 a.a.        Molecular weight: 69079.67 Da        Isoelectric Point: 4.6680

>NTDB_id=57819 ECBG_RS09900 WP_015509978.1 2157320..2159137(-) (pepF) [Enterococcus casseliflavus EC20]
MSETIQLPARESIPVEKTWDLTKIYADDAAFDIEFQEIQAQVKEASRYQGTLADGPQAFLSALEFVLALSRRLEKLYVYS
HLKNDQDTANTTYQGLYAKASNLVAQVSEAVAWFDPELLTMSDETIWGYFDQEPKLAVYRHMIEKTINERPHVLPAEQEA
LLAGAGEIFGAPGNIFSVLNNADLDFPVVEDENGEKVQLSHGVYGELMESTDRSVREAAFKGLYGVYEQFRNTFAQTLST
NIKAHNFKAKARKFDSARQAALSANHIPESVYDTLVDVVNQHLPLLHRYMALRKRLLAVDTLHMYDVYTPILGEAPIRYT
YEEAVEKAKEALKPLGEEYLAVVEEAFSNRWIDVVENKGKRSGAYSSGAYDTLPYILMNWNDRLDQLFTLVHEMGHSVHS
YFTRNNQPYAYGDYSIFLAEIASTTNENILTEYMLQTETDPRVKAYVLNHFLDGFKGTIFRQTQFAEFEHFMHVEDAKGT
PLTSEFLSENYAELNAKYYGPSVEKDPEIRLEWSRIPHFYYNYYVYQYSTGFSAASALAKKILAGEPDALTNYLNYLKAG
RSDYPIEVMKKAGVDMTKADYIEDAMKVFEQRLNELEELVKEIEA

Nucleotide


Download         Length: 1818 bp        

>NTDB_id=57819 ECBG_RS09900 WP_015509978.1 2157320..2159137(-) (pepF) [Enterococcus casseliflavus EC20]
ATGTCAGAAACAATACAATTACCAGCACGTGAATCGATTCCAGTCGAAAAAACGTGGGACTTAACGAAAATCTATGCCGA
TGATGCGGCTTTTGACATAGAATTTCAAGAGATCCAAGCACAAGTCAAAGAAGCAAGTCGTTACCAAGGTACTTTAGCCG
ATGGGCCGCAAGCCTTCTTATCCGCATTAGAATTTGTTTTAGCGCTTTCTCGTCGTTTAGAGAAACTCTATGTATACAGT
CATTTGAAAAACGACCAAGATACTGCCAATACGACGTATCAAGGCTTGTATGCAAAAGCAAGTAACTTGGTTGCGCAAGT
AAGTGAAGCCGTTGCTTGGTTTGATCCTGAGCTATTGACAATGTCTGATGAAACGATTTGGGGCTATTTCGATCAAGAGC
CGAAACTAGCTGTTTATCGTCATATGATCGAAAAAACCATCAACGAGCGTCCACATGTCTTGCCAGCGGAGCAAGAAGCG
TTATTAGCCGGTGCTGGCGAAATTTTTGGGGCACCTGGCAACATTTTTTCTGTTTTAAACAATGCTGATTTGGATTTTCC
AGTAGTTGAAGACGAAAACGGGGAAAAAGTACAGTTATCTCATGGTGTATACGGTGAGTTGATGGAAAGCACGGATCGGT
CTGTCCGTGAAGCAGCCTTTAAAGGTCTTTATGGTGTCTATGAGCAATTCCGCAATACCTTTGCCCAAACATTAAGCACC
AATATCAAAGCCCATAACTTTAAAGCAAAAGCCCGCAAATTTGATTCGGCACGGCAAGCCGCTTTGAGTGCCAACCACAT
TCCTGAAAGTGTCTACGATACATTAGTGGATGTCGTCAATCAGCACCTGCCATTGTTGCATCGTTATATGGCATTAAGAA
AACGCCTGTTGGCTGTCGATACGTTGCATATGTATGATGTTTATACGCCGATTTTAGGGGAAGCACCCATTCGATATACC
TATGAAGAAGCGGTCGAAAAAGCGAAAGAAGCGTTAAAACCATTAGGTGAAGAATACCTTGCGGTGGTTGAAGAAGCCTT
CAGCAATCGGTGGATCGATGTCGTTGAAAACAAAGGCAAACGCAGTGGCGCGTATTCTTCTGGTGCCTATGATACGCTGC
CATATATCCTGATGAACTGGAATGATCGATTGGATCAACTCTTTACTCTTGTTCATGAAATGGGACACAGTGTGCATAGT
TACTTTACACGTAACAACCAGCCTTATGCCTATGGGGATTATTCGATCTTTTTAGCGGAAATCGCTTCAACCACCAATGA
AAATATTTTGACAGAGTATATGCTGCAGACTGAGACAGATCCACGGGTCAAAGCTTATGTCTTGAATCATTTTCTCGATG
GCTTCAAAGGAACGATTTTCCGTCAAACGCAATTTGCCGAGTTTGAACACTTCATGCACGTGGAAGATGCGAAAGGAACG
CCATTGACCAGTGAGTTCTTAAGCGAGAACTATGCCGAGTTAAATGCAAAATACTACGGACCTTCTGTTGAAAAAGATCC
TGAGATTCGTCTTGAGTGGTCACGCATTCCGCATTTCTATTACAATTACTATGTTTACCAGTATTCAACCGGATTTTCGG
CAGCTTCTGCTTTAGCGAAGAAAATCTTAGCAGGTGAACCAGATGCCTTAACCAACTATTTGAACTATTTGAAAGCTGGA
CGCAGTGATTATCCAATCGAAGTCATGAAAAAAGCGGGCGTCGATATGACAAAAGCAGATTATATTGAAGACGCGATGAA
AGTATTTGAGCAACGCTTGAATGAATTGGAAGAACTCGTCAAAGAAATTGAAGCATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB M9T6H0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

56.492

98.017

0.554

Multiple sequence alignment