Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ECBG_RS00115 Genome accession   NC_020995
Coordinates   25008..26378 (+) Length   456 a.a.
NCBI ID   WP_005226394.1    Uniprot ID   A0A242CQN0
Organism   Enterococcus casseliflavus EC20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20008..31378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECBG_RS00085 (ECBG_01165) - 20474..21292 (+) 819 WP_005232914.1 ZIP family metal transporter -
  ECBG_RS00090 (ECBG_01166) - 21348..21551 (-) 204 WP_010748476.1 hypothetical protein -
  ECBG_RS00095 (ECBG_01167) - 21702..22103 (+) 402 WP_015508682.1 DUF4809 family protein -
  ECBG_RS00100 (ECBG_01168) - 22207..23451 (-) 1245 WP_015508683.1 glutamate-5-semialdehyde dehydrogenase -
  ECBG_RS00105 (ECBG_01169) proB 23448..24263 (-) 816 WP_005226390.1 glutamate 5-kinase -
  ECBG_RS00110 (ECBG_01170) - 24454..24963 (+) 510 WP_010748479.1 hypothetical protein -
  ECBG_RS00115 (ECBG_01171) radA 25008..26378 (+) 1371 WP_005226394.1 DNA repair protein RadA Machinery gene
  ECBG_RS16830 - 26389..26511 (+) 123 WP_016608944.1 hypothetical protein -
  ECBG_RS00120 (ECBG_01172) - 26547..27686 (+) 1140 WP_005226396.1 PIN/TRAM domain-containing protein -
  ECBG_RS00125 (ECBG_01173) gltX 27856..29313 (+) 1458 WP_015508684.1 glutamate--tRNA ligase -
  ECBG_RS00130 (ECBG_01174) epsC 29574..30110 (+) 537 WP_026008130.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49604.28 Da        Isoelectric Point: 8.3034

>NTDB_id=57803 ECBG_RS00115 WP_005226394.1 25008..26378(+) (radA) [Enterococcus casseliflavus EC20]
MAKKAKSQFVCQNCGYSSPKYLGKCPNCGSWNSMVEEVIQETADRRVRTTLTGQKMQPTKIADVVPKKEPRVQTKLAELN
RVLGGGVVPGSLILIGGDPGIGKSTLLLQVSQQLAAIGGTVLYVSGEESAEQIKMRAQRLGAIDTEFYLYGETDMSEISK
AIENIRPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHSFRILRAVKNRFGSTNEIGIFEMEEGGLKEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTSPTECFIGEIGLTG
EIRRVNAIEQRVKEVQKLGFTKVYLPKNNLTGWQPPKGIEVEGVSTLAETLRKVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=57803 ECBG_RS00115 WP_005226394.1 25008..26378(+) (radA) [Enterococcus casseliflavus EC20]
ATGGCAAAAAAAGCAAAAAGTCAATTTGTCTGTCAAAATTGTGGGTATAGTTCCCCTAAATATTTAGGGAAATGCCCCAA
TTGCGGCAGCTGGAATTCAATGGTGGAAGAAGTGATCCAAGAAACCGCCGATCGTCGAGTACGTACGACATTGACGGGGC
AAAAGATGCAGCCGACGAAAATCGCCGATGTCGTACCGAAAAAAGAACCGCGGGTCCAAACGAAACTCGCTGAGTTGAAT
CGCGTCTTAGGTGGCGGCGTGGTCCCAGGCTCCTTGATTTTGATCGGTGGGGATCCAGGGATCGGAAAATCGACGCTGCT
CTTGCAAGTTTCCCAGCAATTAGCAGCGATCGGTGGGACGGTCTTGTATGTGTCAGGAGAAGAAAGTGCCGAGCAGATCA
AAATGCGGGCGCAGCGCTTAGGCGCCATCGACACGGAATTTTATCTCTATGGGGAAACGGACATGTCAGAGATCAGTAAA
GCGATCGAAAACATCCGCCCAGATTATGTGATCATCGATTCGATCCAAACCATGACCCAACCGGATATCACCAGCGTGGC
AGGCAGTGTCAGCCAAGTGCGAGAAACGACCGCAGAATTGCTGAAAATCGCCAAAACCAATGGCATCGCCATCTTTATCG
TTGGGCACGTGACGAAGGAAGGCTCGATCGCAGGACCGCGGATGCTGGAGCATATGGTGGATACCGTCTTGTATTTCGAA
GGCGAAAAGCACCACAGTTTTCGAATTTTGCGGGCCGTCAAAAATCGCTTTGGGTCCACCAATGAGATCGGGATTTTTGA
AATGGAAGAAGGCGGGTTGAAAGAAGTCGCCAATCCGTCACAAGTCTTTTTGGAAGAACGCTTAGAAGGCGCAACCGGAT
CGGCGATCGTAGTAGCAATGGAAGGCAGTCGGCCAATCTTGGTGGAGATCCAAGCACTGGTCACGCCAACGATGTTCGGC
AATGCCAAACGCACGACGACGGGGCTAGACTTCAACCGCGTCTCGCTGATCATGGCGGTTTTAGAAAAACGGGCAGGCTT
GCTGCTGCAAAATCAAGATGCTTACTTAAAAGCAGCCGGCGGGGTCAAATTAAATGAACCCGCGATCGATCTAGCGATCG
CTGTTAGTATCGCGTCTAGCTACAAAGAAAAAGGCACCAGCCCGACGGAGTGTTTTATCGGTGAAATTGGCTTGACGGGT
GAGATTCGCCGAGTCAACGCGATCGAGCAACGGGTCAAAGAGGTGCAAAAACTAGGTTTTACGAAAGTTTATTTGCCGAA
AAATAATCTGACTGGCTGGCAGCCGCCCAAAGGCATCGAAGTCGAAGGCGTGTCTACCTTAGCCGAAACCTTGCGGAAAG
TCTTCCGTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A242CQN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.725

99.781

0.746
  radA Streptococcus mitis SK321

74.505

99.781

0.743
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Bacillus subtilis subsp. subtilis str. 168

67.184

98.904

0.664

Multiple sequence alignment