Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KP952_RS13095 Genome accession   NZ_CP076731
Coordinates   2455070..2455480 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain Miz-8     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2450070..2460480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP952_RS13065 (KP952_13065) - 2450158..2450298 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  KP952_RS13070 (KP952_13070) yqeF 2450604..2451335 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  KP952_RS13075 (KP952_13075) cwlH 2451587..2452339 (-) 753 WP_069837642.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  KP952_RS13080 (KP952_13080) yqeD 2452526..2453152 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  KP952_RS13085 (KP952_13085) gnd 2453171..2454064 (-) 894 WP_069837643.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  KP952_RS13090 (KP952_13090) yqeB 2454315..2455037 (+) 723 WP_014480321.1 hypothetical protein -
  KP952_RS13095 (KP952_13095) nucA/comI 2455070..2455480 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  KP952_RS13100 (KP952_13100) sigK 2455676..2456404 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  KP952_RS13105 (KP952_13105) - 2456404..2456502 (+) 99 WP_031600702.1 hypothetical protein -
  KP952_RS21940 - 2456499..2456711 (-) 213 Protein_2557 recombinase family protein -
  KP952_RS13115 (KP952_13115) fumC 2456930..2458318 (-) 1389 WP_014480325.1 class II fumarate hydratase -
  KP952_RS13120 (KP952_13120) - 2458485..2459375 (+) 891 WP_014480326.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=577772 KP952_RS13095 WP_009967785.1 2455070..2455480(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain Miz-8]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=577772 KP952_RS13095 WP_009967785.1 2455070..2455480(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain Miz-8]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529