Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KP952_RS01960 Genome accession   NZ_CP076731
Coordinates   368785..369234 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis subsp. subtilis strain Miz-8     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 363785..374234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP952_RS01920 (KP952_01920) - 364125..364322 (+) 198 WP_121509395.1 hypothetical protein -
  KP952_RS01925 (KP952_01925) yckC 364342..364797 (+) 456 WP_014478793.1 RDD family protein -
  KP952_RS01930 (KP952_01930) yckD 364879..365211 (+) 333 WP_014478794.1 YckD family protein -
  KP952_RS01935 (KP952_01935) - 365339..365761 (+) 423 WP_014478795.1 MarR family winged helix-turn-helix transcriptional regulator -
  KP952_RS01940 (KP952_01940) - 365749..366069 (+) 321 WP_014478796.1 DUF3147 family protein -
  KP952_RS01945 (KP952_01945) - 366088..366817 (+) 730 Protein_341 AAA family ATPase -
  KP952_RS01950 (KP952_01950) bglC 366890..368323 (+) 1434 WP_014478799.1 6-phospho-beta-glucosidase -
  KP952_RS01955 (KP952_01955) nin/comJ 368360..368758 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  KP952_RS01960 (KP952_01960) nucA/comI 368785..369234 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  KP952_RS01965 (KP952_01965) tlpC 369402..371123 (-) 1722 Protein_345 methyl-accepting chemotaxis protein TlpC -
  KP952_RS01970 (KP952_01970) hxlB 371234..371791 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  KP952_RS01975 (KP952_01975) hxlA 371797..372429 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KP952_RS01980 (KP952_01980) hxlR 372658..373020 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=577729 KP952_RS01960 WP_014475770.1 368785..369234(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain Miz-8]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=577729 KP952_RS01960 WP_014475770.1 368785..369234(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain Miz-8]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987