Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MQC90_RS13940 Genome accession   NZ_CP094361
Coordinates   2647604..2648014 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain NCIB 3610     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2642604..2653014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQC90_RS13910 - 2642691..2642831 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  MQC90_RS13915 yqeF 2643137..2643868 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  MQC90_RS13920 cwlH 2644120..2644872 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  MQC90_RS13925 yqeD 2645059..2645685 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  MQC90_RS13930 gnd 2645704..2646597 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  MQC90_RS13935 yqeB 2646849..2647571 (+) 723 WP_010886572.1 hypothetical protein -
  MQC90_RS13940 nucA/comI 2647604..2648014 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  MQC90_RS13945 - 2648210..2648557 (+) 348 Protein_2700 sigma-70 family RNA polymerase sigma factor -
  MQC90_RS13950 spoIVCA 2648588..2650048 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  MQC90_RS13955 - 2650006..2650184 (-) 179 Protein_2702 hypothetical protein -
  MQC90_RS13960 arsC 2650539..2650958 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  MQC90_RS13965 acr3 2650970..2652010 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  MQC90_RS13970 yqcK 2652033..2652473 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  MQC90_RS13975 arsR 2652534..2652851 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=577686 MQC90_RS13940 WP_009967785.1 2647604..2648014(-) (nucA/comI) [Bacillus subtilis strain NCIB 3610]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=577686 MQC90_RS13940 WP_009967785.1 2647604..2648014(-) (nucA/comI) [Bacillus subtilis strain NCIB 3610]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529