Detailed information NTG0577603

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: MQC90_RS02875
• Genome accession: NZ_CP094361
• Coordinates: 546903..548078 (+)
• Length: 391 a.a.
• NCBI ID: WP_009966637.1
• Uniprot ID: P96649
• Organism: Bacillus subtilis strain NCIB 3610
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 541903..553078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MQC90_RS02855	cwlT	543619..544608 (+)	990	WP_009966633.1	bifunctional lytic transglycosylase/C40 family peptidase	-
MQC90_RS02860	-	544623..545129 (+)	507	WP_009966634.1	hypothetical protein	-
MQC90_RS02865	yddJ	545192..545572 (+)	381	WP_009969670.1	DUF4467 domain-containing protein	-
MQC90_RS02870	yddK	545763..546563 (-)	801	WP_009966636.1	toll/interleukin-1 receptor domain-containing protein	-
MQC90_RS02875	rapF	546903..548078 (+)	1176	WP_009966637.1	response regulator aspartate phosphatase RapI	Regulator
MQC90_RS02880	phrI	548035..548154 (+)	120	WP_010886415.1	phosphatase RapI inhibitor PhrI	-
MQC90_RS02885	yddM	548307..549248 (+)	942	WP_010886416.1	helicase	-
MQC90_RS02890	yddN	549837..550856 (-)	1020	WP_003234259.1	LLM class flavin-dependent oxidoreductase	-
MQC90_RS02895	lrpA	551116..551526 (+)	411	WP_003246601.1	transcriptional regulator LrpA	-
MQC90_RS02900	lrpB	551649..552098 (-)	450	WP_003246612.1	transcriptional regulator LrpB	-
MQC90_RS02905	yddQ	552213..552755 (+)	543	WP_003234256.1	cysteine hydrolase family protein	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 46566.15 Da
• Isoelectric Point: 4.9688

>NTDB_id=577603 MQC90_RS02875 WP_009966637.1 546903..548078(+) (rapF) [Bacillus subtilis strain NCIB 3610]
MRGVFLDKDKIPYDLVTKKLNEWYTSIKNDQVEQAEIIKTEVEKELLNMEENQDALLYYQLLEFRHEIMLSYMKSKEIED
LNNAYETIKEIEKQGQLTGMLEYYFYFFKGMYEFRRKELISAISAYRIAESKLSEVEDEIEKAEFFFKVSYVYYYMKQTY
FSMNYANRALKIFREYEEYAVQTVRCQFIVAGNLIDSLEYERALEQFLKSLEISKESNIEHLIAMSHMNIGICYDELKEY
KKASQHLILALEIFEKSKHSFLTKTLFTLTYVEAKQQNYNVALIYFRKGRFIADKSDDKEYSAKFKILEGLFFSDGETQL
IKNAFSYLASRKMFADVENFSIEVADYFHEQGNLMLSNEYYRMSIEARRKIKKGEIIDENQPDSIGSSDFK

Nucleotide

• Length: 1176 bp

>NTDB_id=577603 MQC90_RS02875 WP_009966637.1 546903..548078(+) (rapF) [Bacillus subtilis strain NCIB 3610]
TTGCGGGGTGTTTTCTTAGATAAAGATAAAATTCCGTACGACTTAGTCACGAAAAAGTTAAATGAATGGTATACATCAAT
AAAAAATGATCAAGTTGAGCAAGCCGAGATTATAAAAACAGAAGTAGAGAAAGAATTGTTAAACATGGAAGAAAATCAAG
ATGCCCTGTTATATTATCAACTATTAGAATTTAGACATGAGATAATGCTGAGTTATATGAAATCTAAGGAAATAGAAGAT
CTCAATAATGCTTATGAGACTATAAAAGAAATTGAGAAGCAAGGGCAATTAACTGGCATGTTGGAATACTATTTTTACTT
TTTTAAGGGTATGTACGAGTTTAGGCGTAAAGAATTAATTTCAGCGATAAGTGCTTATCGAATAGCTGAATCAAAGTTGT
CAGAAGTTGAGGATGAAATAGAGAAAGCAGAGTTTTTTTTCAAAGTGTCCTATGTATATTATTATATGAAACAAACATAC
TTCTCCATGAATTATGCAAATCGTGCACTCAAAATATTTAGAGAGTATGAAGAATATGCTGTCCAGACTGTGCGTTGTCA
ATTTATTGTAGCAGGAAACTTGATCGATTCATTGGAATATGAAAGAGCCTTGGAACAATTTTTGAAGTCTTTGGAAATTT
CCAAGGAAAGTAACATAGAGCATTTAATTGCAATGTCACATATGAATATTGGGATTTGTTATGATGAATTGAAAGAATAT
AAGAAGGCTTCACAACATTTAATTTTAGCGTTAGAAATTTTTGAAAAATCAAAACATAGTTTCTTAACAAAGACTTTATT
CACTCTAACCTATGTAGAAGCAAAACAACAAAATTATAATGTTGCTTTGATATACTTTAGGAAAGGGCGATTTATTGCCG
ATAAAAGTGATGATAAGGAATACTCAGCGAAATTCAAAATATTAGAGGGATTATTTTTTTCTGATGGTGAGACTCAATTA
ATAAAGAATGCATTTTCATATCTGGCTTCGAGAAAAATGTTTGCTGATGTTGAAAATTTTTCGATTGAAGTCGCTGATTA
TTTTCATGAACAAGGAAATTTAATGCTCTCTAATGAATATTATCGTATGAGTATTGAAGCAAGACGAAAAATTAAAAAAG
GGGAGATTATTGATGAAAATCAGCCGGATTCTATTGGCAGCAGTGATTTTAAGTAG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (10-70)

• Tetratricopeptide repeat (TPR_12) (199-256)

3D structure

• PDB: 4I1A

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	38.525	93.606	0.361
rapC	Bacillus subtilis subsp. subtilis str. 168	38.108	94.629	0.361