Detailed information NTG0057759

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: CCX78_RS05140
• Genome accession: NZ_AP017931
• Coordinates: 1003031..1006774 (-)
• Length: 1247 a.a.
• NCBI ID: WP_056947675.1
• Uniprot ID: -
• Organism: Latilactobacillus sakei strain LK-145
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 998031..1011774

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CCX78_RS05125 (LASAK_01021)	asnS	998169..999467 (-)	1299	WP_011374616.1	asparagine--tRNA ligase	-
CCX78_RS05130 (LASAK_01022)	-	999578..1000030 (-)	453	WP_231952313.1	DUF5590 domain-containing protein	-
CCX78_RS05135 (LASAK_01023)	-	1000131..1002965 (-)	2835	WP_056947673.1	helicase C-terminal domain-containing protein	-
CCX78_RS05140 (LASAK_01024)	addA	1003031..1006774 (-)	3744	WP_056947675.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
CCX78_RS05145 (LASAK_01025)	-	1006758..1010318 (-)	3561	WP_056947677.1	PD-(D/E)XK nuclease family protein	-
CCX78_RS05150 (LASAK_01026)	mvk	1010581..1011546 (+)	966	WP_231952314.1	mevalonate kinase	-

Sequence

Protein

• Length: 1247 a.a.
• Molecular weight: 141766.78 Da
• Isoelectric Point: 4.8355

>NTDB_id=57759 CCX78_RS05140 WP_056947675.1 1003031..1006774(-) (addA) [Latilactobacillus sakei strain LK-145]
MAILSNPQFTPSQQAAVNHDGHDILVSASAGSGKTSVLVARVIQKILKGTDVDTLLVVTFTEAAATEMRQRIQAALRDAS
EKATEPAVKQRLRQQLSLVPTAQISTLHAFCLKVIKQFYYVIDRDPVFRLLSDTAERLLLADQVWQRVREAFYSHEYVEK
GTQDTLFYELAQNFSNDRNDDGLTDIVFELLDFANANPNPVKWLNELPKSYAVDETGLTASRYFQEKILPILQQTLTECL
EALQEAEQLSQTSENLMVYAPQITTTQAALTQIKADAKTLNWQAWRQQLTDAQLGPAKRTKKLEPDEQINKDRLKADLDD
VKKKIQTLLDTYFVFDEATTTNIQRQAGQLVQKLVRVTLTFREAFQAEKERRHLLDFSDLEQLCLTILSAEDSPARAFYQ
QKFSEVLVDEYQDTNPLQETIIQKVTSDHPRNLFMVGDVKQSIYAFRLADPSLFKNKYNEFGVTEAERDSERIILAENFR
SRRNIDDFTNLIFKQLMDENLGELDYDENAALQYGARYYPDQHPTAPTELLLYETKPEEAVANPQLDKSEGQVVAVAKRI
QAMMTNGEQIWDKKLEKMRPIEYRDIVLLAPTRGNNLFILDYFKRFGLPVVIKDAQNYFQTTEVQIMLALLQVIDNPNQD
IPLVSVLRSPIVGLNENELALIRINDKTDDYYQAVFNFIDQYNEQKVGHLGQQVMQKLTHFMALLTSFRTIARQQPIVDL
IWTIYQETGFLDYVGGMPAGRQRQANLHALYERATAYEENGFKGLFQFIQFIERLQKQDKDLAQPTSLENQDAISVMTIH
GSKGLEFPVVFLIDTSRRFNQQDLQRSYVLDNHGGLGVVYLDSQKRLKVPTLPELAITAQKRKKLRAEEMRKLYVALTRA
EQWLIIVGHCDNQAQLMKQWQKGQNSTHQILDDGVRNDAASLLDWIGLSLIRHPQAKDWITDYEPLLALSGDQTEFKLIM
TNEALLGEIPGSLHNDQDWGQQQQKALQTIDLNSGIATAIAEQLTFKYPANVATQTTAYQSVSEVKRLFEDPDNTGLGQL
DLAAEQPRQANRYVTDSLAQPQFLQTVQQPTAAEVGTATHLVLQEIDLTQPVTLASLQGLVDRLVLQNSLSANVAAKINL
NHLLTFFESDLGQQLLAHPEAVKREVPFSLLLPAETIFEGIRPTDDDHVLIHGIMDGYLLTDDGCYLFDYKTDFIDPSDQ
EAAIERVKNRYAGQINLYSAALNQIVQKPVTHKYLYLLSIGELVEIN

Nucleotide

• Length: 3744 bp

>NTDB_id=57759 CCX78_RS05140 WP_056947675.1 1003031..1006774(-) (addA) [Latilactobacillus sakei strain LK-145]
ATGGCAATCTTGAGTAACCCGCAATTTACACCTTCACAGCAAGCGGCTGTCAATCATGACGGCCACGATATTTTAGTATC
CGCATCAGCTGGTTCAGGGAAGACATCCGTCCTGGTTGCCCGGGTCATCCAGAAAATCTTAAAAGGCACCGATGTCGACA
CCTTGCTTGTTGTGACTTTTACGGAAGCCGCTGCTACTGAAATGCGCCAACGGATTCAAGCCGCACTACGCGATGCCAGT
GAGAAAGCGACCGAGCCAGCTGTTAAACAACGTTTACGGCAACAACTGAGTCTCGTGCCAACCGCACAAATCAGTACGCT
GCATGCTTTTTGTTTAAAAGTCATTAAACAATTTTATTACGTGATTGACCGTGATCCCGTATTCCGCTTATTGAGTGATA
CTGCAGAACGACTCCTCTTAGCGGATCAAGTATGGCAACGGGTGAGAGAAGCATTCTACAGCCACGAGTATGTTGAAAAG
GGCACCCAAGACACCTTATTTTACGAATTGGCCCAAAATTTCTCGAATGATCGTAACGATGACGGCCTAACCGACATTGT
TTTTGAGTTGCTCGATTTTGCAAATGCCAATCCAAACCCGGTTAAATGGTTAAACGAACTACCCAAAAGCTATGCTGTTG
ATGAGACTGGTTTGACTGCCAGTCGCTATTTCCAAGAAAAAATATTACCGATATTACAGCAAACACTCACCGAATGCTTA
GAAGCTTTACAAGAAGCTGAACAGCTCAGTCAAACTAGCGAAAATCTGATGGTTTATGCGCCTCAAATTACCACTACGCA
AGCAGCCTTAACACAAATCAAAGCAGATGCCAAAACGTTAAATTGGCAAGCATGGCGCCAACAACTAACGGATGCCCAGC
TAGGACCTGCTAAACGCACTAAGAAATTAGAGCCTGATGAACAAATTAATAAAGACCGTCTGAAAGCCGATTTAGATGAT
GTCAAAAAGAAAATCCAAACGTTATTAGACACTTATTTTGTGTTTGATGAAGCAACCACCACTAATATTCAGCGCCAAGC
TGGCCAGTTGGTCCAAAAATTGGTGCGCGTCACGTTAACTTTCAGAGAAGCCTTTCAAGCCGAAAAAGAACGACGCCATT
TATTGGACTTTAGTGATTTAGAACAACTCTGTCTGACAATTTTATCGGCAGAAGATTCACCAGCAAGAGCCTTTTACCAA
CAAAAATTTAGCGAAGTGCTAGTTGATGAATACCAAGATACCAATCCGCTCCAAGAAACGATTATCCAAAAGGTCACTTC
TGATCATCCACGCAATCTCTTCATGGTCGGGGATGTTAAGCAGTCAATTTATGCTTTCCGATTGGCCGATCCAAGCTTAT
TTAAGAATAAGTATAACGAATTTGGTGTCACGGAAGCTGAACGTGATAGTGAACGAATCATCTTAGCTGAAAACTTCCGT
TCGCGCCGCAATATCGATGACTTTACCAATCTGATTTTTAAACAATTAATGGACGAAAATCTTGGTGAATTAGACTATGA
CGAAAATGCCGCCTTACAATATGGTGCTCGTTATTATCCTGACCAGCACCCAACCGCGCCAACCGAACTCTTGCTTTATG
AAACTAAGCCGGAAGAGGCAGTTGCCAACCCGCAATTAGATAAGAGTGAGGGCCAAGTCGTGGCGGTCGCTAAACGGATT
CAAGCCATGATGACTAATGGGGAACAGATTTGGGATAAGAAACTCGAAAAAATGCGGCCGATTGAATATCGGGACATCGT
TTTACTAGCCCCAACCCGCGGTAATAATTTGTTTATCCTGGATTACTTTAAACGCTTCGGCTTGCCGGTTGTGATTAAGG
ATGCGCAAAATTATTTCCAAACGACTGAAGTCCAAATTATGTTAGCCCTACTTCAGGTGATTGATAATCCTAACCAAGAT
ATTCCACTGGTCAGTGTTTTACGCTCACCAATTGTCGGGTTAAACGAAAATGAATTGGCATTAATTCGGATTAACGATAA
GACGGATGATTATTATCAAGCGGTCTTTAACTTTATTGACCAATATAATGAGCAGAAAGTCGGCCACTTAGGCCAACAAG
TGATGCAGAAGCTGACGCACTTCATGGCTTTATTGACGTCGTTTAGAACAATCGCTCGTCAACAACCGATTGTGGACTTA
ATTTGGACGATCTACCAAGAAACTGGCTTTTTAGATTATGTCGGGGGGATGCCAGCTGGACGTCAACGTCAAGCTAATTT
ACACGCGCTTTATGAACGTGCCACGGCGTATGAAGAAAATGGTTTTAAAGGACTCTTCCAGTTCATTCAGTTTATCGAAC
GTCTTCAAAAACAAGATAAGGATTTAGCACAACCAACGAGCTTAGAAAATCAAGACGCCATCTCTGTTATGACCATTCAC
GGGAGTAAGGGACTTGAATTTCCAGTCGTCTTTTTAATCGATACTAGTCGCCGCTTTAACCAACAGGATTTACAACGCTC
ATATGTTTTGGATAATCATGGTGGTTTAGGGGTCGTTTATTTAGATAGCCAAAAGCGGTTGAAGGTACCAACACTGCCAG
AATTAGCGATTACCGCCCAGAAACGAAAAAAATTACGAGCAGAAGAAATGCGAAAACTATACGTTGCTTTGACACGTGCG
GAACAATGGTTAATCATCGTTGGCCATTGTGATAACCAGGCCCAATTGATGAAGCAATGGCAAAAAGGGCAAAATAGTAC
CCACCAAATATTAGATGATGGTGTTCGCAACGATGCCGCAAGTTTACTGGACTGGATAGGTCTTAGCTTAATTCGCCATC
CACAAGCTAAAGATTGGATCACTGATTATGAGCCACTACTGGCTTTAAGTGGTGATCAAACCGAGTTTAAGTTGATTATG
ACTAATGAAGCCTTACTTGGTGAAATACCAGGTAGCCTGCATAACGATCAGGACTGGGGGCAACAACAGCAAAAGGCACT
TCAAACAATTGATTTAAACAGTGGGATAGCAACAGCGATTGCTGAGCAATTAACCTTTAAGTATCCCGCTAATGTTGCGA
CCCAAACGACGGCTTATCAATCTGTTTCGGAAGTCAAACGCCTTTTTGAAGACCCTGATAATACCGGATTGGGACAATTA
GACCTAGCGGCTGAACAACCGCGCCAAGCAAATCGCTACGTAACGGATAGCTTAGCGCAACCACAGTTCTTACAAACCGT
TCAACAACCAACGGCAGCAGAAGTGGGGACCGCAACGCATTTGGTCTTGCAAGAAATTGATTTAACGCAACCAGTAACGC
TAGCGAGTTTGCAAGGTCTTGTCGATCGACTTGTTTTACAGAATAGTTTAAGCGCCAATGTTGCGGCTAAGATTAATCTT
AATCACCTATTAACCTTCTTTGAAAGTGACTTGGGACAGCAATTATTAGCCCATCCAGAAGCGGTTAAGCGGGAAGTGCC
ATTTTCGTTGTTATTACCAGCAGAAACGATTTTTGAAGGCATTCGTCCCACAGATGACGACCACGTCCTAATCCATGGGA
TTATGGATGGTTATTTATTGACTGACGACGGTTGTTATTTATTTGATTACAAAACCGACTTTATTGACCCCAGTGATCAA
GAAGCGGCGATTGAACGGGTTAAAAACCGCTATGCTGGCCAGATTAACCTCTATAGCGCTGCTTTAAATCAGATTGTCCA
AAAGCCAGTGACCCATAAATATCTTTATTTATTGTCAATTGGCGAACTGGTTGAGATTAACTAG

Domains

Predicted by InterProScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (473-887)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (10-461)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1067-1225)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	37.52	100	0.383