Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KPL75_RS07925 Genome accession   NZ_CP076653
Coordinates   1503026..1504069 (-) Length   347 a.a.
NCBI ID   WP_219920191.1    Uniprot ID   -
Organism   Bacillus sp. NP247     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1498026..1509069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPL75_RS07880 (KPL75_07875) - 1498394..1498594 (-) 201 WP_002015009.1 YqzE family protein -
  KPL75_RS07885 (KPL75_07880) - 1498633..1499130 (-) 498 WP_219920178.1 shikimate kinase -
  KPL75_RS07890 (KPL75_07885) - 1499314..1499964 (-) 651 WP_219920180.1 2OG-Fe(II) oxygenase -
  KPL75_RS07895 (KPL75_07890) comGG 1500137..1500508 (-) 372 WP_219920182.1 competence type IV pilus minor pilin ComGG -
  KPL75_RS07900 (KPL75_07895) comGF 1500505..1500966 (-) 462 WP_219920184.1 competence type IV pilus minor pilin ComGF -
  KPL75_RS07905 (KPL75_07900) - 1500950..1501246 (-) 297 WP_219921083.1 type II secretion system protein -
  KPL75_RS07910 (KPL75_07905) comGD 1501239..1501694 (-) 456 WP_219920187.1 competence type IV pilus minor pilin ComGD -
  KPL75_RS07915 (KPL75_07910) comGC 1501691..1501990 (-) 300 WP_002088623.1 competence type IV pilus major pilin ComGC -
  KPL75_RS07920 (KPL75_07915) comGB 1502002..1503039 (-) 1038 WP_309137454.1 competence type IV pilus assembly protein ComGB -
  KPL75_RS07925 (KPL75_07920) comGA 1503026..1504069 (-) 1044 WP_219920191.1 competence type IV pilus ATPase ComGA Machinery gene
  KPL75_RS07930 (KPL75_07925) - 1504275..1504970 (+) 696 WP_219920193.1 metalloregulator ArsR/SmtB family transcription factor -
  KPL75_RS07935 (KPL75_07930) - 1505100..1505342 (+) 243 WP_002015110.1 DUF2626 domain-containing protein -
  KPL75_RS07940 (KPL75_07935) - 1505451..1506845 (+) 1395 WP_219920195.1 L-cystine transporter -
  KPL75_RS07945 (KPL75_07940) - 1507089..1507488 (-) 400 Protein_1610 hypothetical protein -
  KPL75_RS07950 (KPL75_07945) - 1507576..1507791 (-) 216 WP_219920197.1 DUF3912 family protein -
  KPL75_RS07955 (KPL75_07950) - 1508044..1508355 (+) 312 WP_219920199.1 hypothetical protein -
  KPL75_RS07960 (KPL75_07955) - 1508389..1508877 (-) 489 WP_219920201.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39019.30 Da        Isoelectric Point: 8.8465

>NTDB_id=576985 KPL75_RS07925 WP_219920191.1 1503026..1504069(-) (comGA) [Bacillus sp. NP247]
MNGIELFANTIMKEACGVQASDLHIVPRQKDVAIQLRIGKDLITKRCIEKEFGKKLVSHFKFLASMDIGERRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSSAEKLLSFLKHSHGLLVFTGPTGSGKTTTMYALLEV
ARKGQTRRIVTLEDPVERRKDGLLQIQINEKAGITYETGLKAILRHDPDIILVGEIRDEETAKVAVRASLTGHLVMTTLH
TNDAKGAILRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQAIKEASGEH
VTYHYETLEASVRKGYALGFLEEDIYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=576985 KPL75_RS07925 WP_219920191.1 1503026..1504069(-) (comGA) [Bacillus sp. NP247]
ATGAATGGTATCGAGCTTTTTGCGAATACGATTATGAAAGAAGCTTGTGGGGTGCAAGCATCGGACTTACATATTGTGCC
CAGGCAGAAGGATGTGGCAATTCAATTACGTATAGGAAAAGATTTAATTACGAAACGGTGTATTGAAAAGGAATTTGGAA
AAAAGCTTGTTTCGCACTTTAAGTTTTTAGCATCAATGGATATAGGAGAGAGGAGAAAACCTCAAAATGGTTCGTTGTAC
TTACAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
CCATTTACAAGCATCTGTTCAGCCATTGTCTCATCTTTCGTTATTTCCAAGTTCAGCGGAAAAATTACTCTCTTTTTTAA
AGCATTCTCATGGATTACTCGTATTTACTGGGCCGACTGGTTCTGGAAAAACAACAACAATGTATGCGTTATTAGAAGTA
GCTAGAAAAGGGCAAACACGTCGCATCGTTACACTTGAAGATCCAGTTGAGAGAAGAAAGGACGGTTTATTACAAATTCA
AATTAATGAAAAAGCAGGTATCACATATGAGACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATATTAGTTG
GTGAAATCCGTGATGAAGAAACAGCAAAAGTAGCTGTAAGAGCAAGTTTGACAGGCCATTTAGTAATGACGACGTTGCAT
ACAAATGACGCGAAAGGGGCGATATTACGATTTATGGATTATGGTATTACAAGGCAAGAAATTGAACAATCATTATTGGC
TGTAGCTGCTCAGCGGCTCGTCGAATTAAAATGTCCGTTTTGCAGAGGGAAGTGTTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCGAGTATTTATGAACTGTTATATGGATATGAATTAAAACAAGCGATTAAAGAAGCAAGCGGAGAACAT
GTTACGTATCACTATGAAACATTGGAAGCATCAGTGCGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATATTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Glaesserella parasuis strain SC1401

43.86

82.133

0.36