Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KPL75_RS06600 Genome accession   NZ_CP076653
Coordinates   1280913..1281347 (-) Length   144 a.a.
NCBI ID   WP_219919869.1    Uniprot ID   -
Organism   Bacillus sp. NP247     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1275913..1286347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPL75_RS06585 (KPL75_06580) - 1277054..1277611 (+) 558 WP_219919863.1 PadR family transcriptional regulator -
  KPL75_RS06590 (KPL75_06585) metE 1278154..1280442 (+) 2289 WP_219919865.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  KPL75_RS06595 (KPL75_06590) comJ 1280484..1280873 (-) 390 WP_219919867.1 competence protein ComJ -
  KPL75_RS06600 (KPL75_06595) nucA/comI 1280913..1281347 (-) 435 WP_219919869.1 DNA-entry nuclease Machinery gene
  KPL75_RS06605 (KPL75_06600) - 1281584..1282327 (+) 744 WP_219919874.1 DUF3967 domain-containing protein -
  KPL75_RS06610 (KPL75_06605) - 1282387..1283661 (-) 1275 WP_219919876.1 HAMP domain-containing sensor histidine kinase -
  KPL75_RS06615 (KPL75_06610) - 1283971..1284171 (+) 201 WP_219919878.1 hypothetical protein -
  KPL75_RS06620 (KPL75_06615) - 1284415..1285578 (+) 1164 WP_219919880.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16180.56 Da        Isoelectric Point: 8.4331

>NTDB_id=576982 KPL75_RS06600 WP_219919869.1 1280913..1281347(-) (nucA/comI) [Bacillus sp. NP247]
MKQFKGIIISIIAILSLLVAVYEVLVPEETSTKKTTTYDQILEFPKERYPETGKHITDAIKDGHSEMCTIDRNGAADRRK
LSLAPYPTKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=576982 KPL75_RS06600 WP_219919869.1 1280913..1281347(-) (nucA/comI) [Bacillus sp. NP247]
ATGAAGCAATTTAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTCTTTTGGTAGCGGTATATGAAGTACTTGTGCC
AGAGGAGACAAGTACTAAAAAAACAACTACGTATGATCAAATTCTAGAGTTCCCGAAAGAACGATATCCAGAGACCGGGA
AACATATAACAGATGCTATAAAAGATGGGCATTCAGAAATGTGTACAATTGATCGTAATGGTGCAGCGGATAGAAGGAAA
TTATCGTTAGCGCCATATCCGACGAAGAAAGGTTATGATCGTGATGAATGGCCGATGGCGATGTGTAAGGAAGGCGGAAA
AGGAGCTCATATTGAATATATAAGTCCAGCAGATAACCGCGGTGCAGGTTCTTGGGTAGGGAATAAGCTAGACAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAATAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(47-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

68.142

78.472

0.535