Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KMZ14_RS12800 Genome accession   NZ_CP076603
Coordinates   2644000..2644800 (-) Length   266 a.a.
NCBI ID   WP_273798031.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain MCDA01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2639000..2649800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ14_RS12780 (KMZ14_12720) - 2640045..2640743 (-) 699 WP_273798028.1 YfgM family protein -
  KMZ14_RS12785 (KMZ14_12725) hisS 2640774..2642069 (-) 1296 WP_273798029.1 histidine--tRNA ligase -
  KMZ14_RS12790 (KMZ14_12730) ispG 2642073..2643194 (-) 1122 WP_008303409.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KMZ14_RS12795 (KMZ14_12735) - 2643218..2644009 (-) 792 WP_273798030.1 helix-turn-helix domain-containing protein -
  KMZ14_RS12800 (KMZ14_12740) pilF 2644000..2644800 (-) 801 WP_273798031.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KMZ14_RS12805 (KMZ14_12745) rlmN 2644815..2646050 (-) 1236 WP_273798032.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KMZ14_RS12810 (KMZ14_12750) ndk 2646173..2646604 (-) 432 WP_273798033.1 nucleoside-diphosphate kinase -
  KMZ14_RS12815 (KMZ14_12755) iscX 2646715..2646915 (-) 201 WP_004813114.1 Fe-S cluster assembly protein IscX -
  KMZ14_RS12820 (KMZ14_12760) - 2647005..2647556 (-) 552 WP_004813115.1 phosphatase PAP2 family protein -
  KMZ14_RS12825 (KMZ14_12765) - 2647626..2648036 (-) 411 WP_004890015.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30172.44 Da        Isoelectric Point: 9.5504

>NTDB_id=576602 KMZ14_RS12800 WP_273798031.1 2644000..2644800(-) (pilF) [Acinetobacter schindleri strain MCDA01]
MRNLTSKIAFISTVCVSIALSGCQTPEVGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDEALKINSRDAQANMMMGVL
LQQEGSKLNLDRAETYFKRAISVEPKNAQARNNYGTYLYQLERYNEAIEQFAIAGATLGYDQRYLALENMGRIYLKQGNL
VNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAASNMYQQFVRSVGQKNQGARALWIGIRTARAEGDQMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=576602 KMZ14_RS12800 WP_273798031.1 2644000..2644800(-) (pilF) [Acinetobacter schindleri strain MCDA01]
TTGAGAAATCTGACATCTAAAATTGCTTTTATTTCAACAGTATGTGTTTCTATTGCTCTATCTGGCTGTCAAACACCAGA
AGTGGGTAAAAAGGATCCTAAAAAAGCGACCCAGGTGCGTACTCAATTGGCGGTTGAATATCTGAAGACTGGTGATCTGG
ATTCAGCGAAAAGAGCGCTAGATGAGGCGCTTAAAATTAATTCACGTGATGCTCAAGCCAATATGATGATGGGTGTTTTG
TTACAGCAGGAAGGCAGTAAGCTGAACTTGGATCGTGCCGAAACTTATTTTAAGCGCGCGATTTCAGTCGAACCTAAAAA
TGCACAGGCACGTAACAATTATGGCACCTATTTGTATCAGCTTGAACGTTATAATGAGGCAATTGAACAGTTTGCAATTG
CAGGCGCGACACTGGGCTATGACCAGCGTTATCTTGCGCTTGAAAATATGGGACGGATCTACCTGAAGCAGGGCAATCTG
GTGAATGCTGAAAAATCATTCAAACAGGCCCTGCAAGCCAATCGCGATTCTTACATTTCAATGTTAGAGTTGGCAGAGAT
TTTTTATCTGAATCAGCAGTTCCCGGCAGCGTCGAATATGTATCAACAATTTGTACGTTCAGTGGGACAGAAAAACCAGG
GTGCTCGTGCGCTCTGGATCGGCATTCGTACTGCGCGTGCCGAGGGTGATCAAATGGGCATGCAAGTATTGGTCAATCAA
TTGCGCGCATTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_2)

(146-176)

Tetratricopeptide repeat (TPR_16)

(89-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.692

97.744

0.759
  pilF Acinetobacter baylyi ADP1

68.75

96.241

0.662