Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   KPG64_RS06255 Genome accession   NZ_CP076539
Coordinates   1198357..1200183 (+) Length   608 a.a.
NCBI ID   WP_000003356.1    Uniprot ID   A0A9X1AZI2
Organism   Bacillus thuringiensis strain GR007     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1193357..1205183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPG64_RS06235 (KPG64_06235) - 1193660..1194334 (-) 675 WP_000362600.1 TerC family protein -
  KPG64_RS06240 (KPG64_06240) mecA 1194680..1195363 (+) 684 WP_000350716.1 adaptor protein MecA Regulator
  KPG64_RS06245 (KPG64_06245) - 1195436..1196980 (+) 1545 WP_000799189.1 cardiolipin synthase -
  KPG64_RS06250 (KPG64_06250) - 1197061..1198305 (+) 1245 WP_000628322.1 competence protein CoiA -
  KPG64_RS06255 (KPG64_06255) pepF 1198357..1200183 (+) 1827 WP_000003356.1 oligoendopeptidase F Regulator
  KPG64_RS06260 (KPG64_06260) - 1200315..1200488 (-) 174 WP_000750354.1 hypothetical protein -
  KPG64_RS06265 (KPG64_06265) - 1200718..1201611 (-) 894 WP_000360643.1 ClpXP adapter SpxH family protein -
  KPG64_RS06270 (KPG64_06270) - 1201611..1202009 (-) 399 WP_000043386.1 hypothetical protein -
  KPG64_RS06275 (KPG64_06275) - 1202178..1202756 (-) 579 WP_000191102.1 CYTH domain-containing protein -
  KPG64_RS06280 (KPG64_06280) - 1202903..1203274 (+) 372 WP_001179996.1 hypothetical protein -
  KPG64_RS06285 (KPG64_06285) - 1203305..1203943 (+) 639 WP_001081480.1 GTP pyrophosphokinase family protein -
  KPG64_RS06290 (KPG64_06290) - 1203962..1204759 (+) 798 WP_000673196.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70176.44 Da        Isoelectric Point: 4.5990

>NTDB_id=576330 KPG64_RS06255 WP_000003356.1 1198357..1200183(+) (pepF) [Bacillus thuringiensis strain GR007]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSVSEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDGRVREDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=576330 KPG64_RS06255 WP_000003356.1 1198357..1200183(+) (pepF) [Bacillus thuringiensis strain GR007]
ATGTCTGAACAAAACAAAGCGAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGACGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGCGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTACCTGAAATTTTGTCGGTTTCAGAAGATACACTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCAGAAGCT
GAGGAGGCTTTATTAGCGGAAGCATCTGAAGTAATGAGTTCATCAAGCAATACATTTGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGTGAAGACGGAGAAGAAATAGAAATTACACATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATGGCCGTGTTCGCGAAGATGCATTTAAAGCTGTATATGAAACGTACGGGAAATTTAAAAATACATTCGCAAGTACG
TTAAGCGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCACTAAGCAA
TAATAATATTCCAGAAGCAGTATATGATCAACTTGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATCG
ATATTCGTAAGCGTGCATTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGATATTTTGAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGGGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAATTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTTACGCCAGACATGCTAACGGAAATCTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAGATTGGTTTAGAATGGTCTCGTATTCCGCACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCTCTATCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAACGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCACTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.916

97.533

0.487