Detailed information NTG0576218

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: JNUCC24_RS05160
• Genome accession: NZ_CP076532
• Coordinates: 1065882..1069586 (+)
• Length: 1234 a.a.
• NCBI ID: WP_185854223.1
• Uniprot ID: -
• Organism: Bacillus sp. JNUCC-24
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1060882..1074586

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JNUCC24_RS05145 (JNUCC24_05145)	-	1061188..1061598 (+)	411	WP_024425497.1	DoxX family protein	-
JNUCC24_RS05150 (JNUCC24_05150)	-	1061796..1062242 (-)	447	WP_024425498.1	ferritin-like domain-containing protein	-
JNUCC24_RS05155 (JNUCC24_05155)	addB	1062395..1065904 (+)	3510	WP_185854222.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
JNUCC24_RS05160 (JNUCC24_05160)	addA	1065882..1069586 (+)	3705	WP_185854223.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
JNUCC24_RS05165 (JNUCC24_05165)	sbcD	1069613..1070788 (+)	1176	WP_216100233.1	exonuclease subunit SbcD	-
JNUCC24_RS05170 (JNUCC24_05170)	-	1070785..1074180 (+)	3396	WP_185854224.1	AAA family ATPase	-
JNUCC24_RS05175 (JNUCC24_05175)	-	1074201..1074500 (+)	300	WP_024425503.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 142301.29 Da
• Isoelectric Point: 5.0100

>NTDB_id=576218 JNUCC24_RS05160 WP_185854223.1 1065882..1069586(+) (addA) [Bacillus sp. JNUCC-24]
MQIPKPNNSTWTDDQWEAIVSEGQDILVAAAAGSGKTAVLVERLIRKMTRPEDPVDVDRLLVVTFTNASAAEMKHRITEA
LEKELAKNPGSLHMRRQLSLMNRANISTLHSFCLQVLRTFYYEIDLDPGFRLADQTEGELLGDEVLDELFEDEYKAGKPA
FFELVDRYTSDRHDLDLQWLVKRIYEFSRSHPSPEQWMRAFLSLYDVDAQTKVEQLPFYPYIKEDLSLVFRSCLELLERA
LALSKEPGGPAPRAENFIDDLEQVNELIRHQDDFEKLYELLPNINFKRLKTCKGDEYDPVLLEKATDARNQAKKQLEKLK
DEYFMRSPAQHLKSLAEMKPVVETLVELVIQFGQRFERAKQEKSIVDFSDLEHYCLRILAEQDAEGNLIETEAAKYYQQQ
FEEVLVDEYQDTNLVQETILKLVSKGEHAAEGNLFMVGDVKQSIYRFRLAEPMLFLNKYKQFTPDGKETGKRIDLNKNFR
SRSDVLDSTNFLFKQLMGETVGEIEYDEQAELKLGASYPESEDTTTEMLLVHLDQQEAETDEEREELETVQLEARVIAKK
IRQLVEQPFQVYDSKQQMTRNLQYRDIVILLRSMPWAPQMMEELKKQGIPVYANLSSGYFEATEVSVILSLLKVIDNPYQ
DIPLAAVLRSPIVHLDENEMALIRTSDKKGTYYDAVKAFMSVTHSDHPTCKKLERFFRLLRKWRDFSMNHSVAELIWEVY
RDTQYLDYVGGMPGGKQRQANLRALYDRAKQYEKAAFRGLFRFLRFIERMQERGDDLGAAKTFSETEDVVRMMTIHSSKG
LEFPVVFTAGLGRNFNMMDLNQSYLLDKELGFGSKYIHPELRISYATLPLVAMKKKMRKELLSEELRVLYVALTRAKEKL
FLVGSVKNQVKALSKWQNAATGEDWLLPDFERYQSKTYLDFIGSALIRHQAMSSILEETGDVVLSHPSAFTISFTQASDL
LKEDMSLEKKQQDEVVQALMDGLPVEGYGDADEKVAERLSWRYPYLAASQVGTKHSVSEIKRMKEIQDEYSVPSSLRRAH
ATLYDRPAFMKKKALTAAEQGTAMHTVMQHIPLSSDEPLDETRIHQLLDSLQQRDLLTNEQVQSIDQEGISAFFYTSIGQ
KLLKADWVKREVSFSMVLPVKEVYSHIDAEGEPVLIQGMIDCLFETDGKLYLLDYKTDRVQGRYTGGLEAAVPILKKRYE
TQIALYAQAVERLTNRTLEEKILYFFDGNLEISL

Nucleotide

• Length: 3705 bp

>NTDB_id=576218 JNUCC24_RS05160 WP_185854223.1 1065882..1069586(+) (addA) [Bacillus sp. JNUCC-24]
ATGCAAATACCAAAACCGAATAACAGTACTTGGACGGATGACCAGTGGGAAGCCATCGTTTCAGAAGGACAAGATATTCT
TGTTGCGGCGGCGGCAGGGTCAGGTAAAACAGCTGTTTTGGTTGAACGGCTGATTCGAAAAATGACCCGTCCTGAAGATC
CAGTTGATGTGGATCGTCTGCTCGTTGTGACTTTCACAAATGCATCTGCGGCAGAGATGAAGCATCGGATCACAGAAGCA
CTTGAAAAGGAATTAGCCAAAAACCCCGGGTCCCTTCATATGAGAAGACAGCTGTCGCTCATGAATCGTGCCAATATTTC
CACCCTGCACTCCTTTTGCTTGCAGGTTTTGCGCACCTTTTACTATGAGATTGACCTAGATCCAGGCTTTCGTTTGGCTG
ATCAAACAGAAGGAGAATTATTAGGAGATGAAGTGTTAGATGAACTGTTTGAGGACGAATATAAGGCTGGAAAGCCAGCG
TTCTTTGAACTGGTTGATCGCTACACTTCTGATCGTCATGATTTAGACTTGCAATGGCTTGTAAAGAGAATTTATGAGTT
TTCCAGATCTCACCCTTCACCAGAGCAATGGATGAGGGCATTCCTTTCTCTTTATGATGTAGATGCTCAAACAAAAGTAG
AACAATTACCGTTTTACCCATATATCAAAGAGGACCTTTCTCTCGTTTTTCGAAGCTGTCTGGAACTGCTCGAACGAGCT
CTCGCCCTATCAAAAGAACCGGGCGGCCCAGCACCGAGAGCAGAAAATTTTATAGATGATTTAGAGCAAGTCAATGAATT
AATCAGGCACCAAGATGATTTTGAGAAGCTTTACGAGCTTTTGCCAAACATCAATTTTAAAAGGCTCAAAACATGTAAAG
GGGACGAATATGACCCTGTGCTGTTAGAAAAAGCCACAGATGCACGCAATCAAGCAAAAAAACAATTAGAAAAGCTTAAA
GATGAATACTTTATGCGCAGTCCGGCCCAGCATTTGAAAAGCTTAGCTGAAATGAAGCCGGTGGTCGAGACACTTGTGGA
GCTTGTCATCCAATTTGGACAGCGTTTCGAAAGAGCGAAGCAAGAAAAATCAATCGTGGATTTTTCGGATTTAGAGCATT
ATTGCTTACGCATTTTAGCAGAGCAGGACGCGGAAGGAAATTTGATCGAAACAGAAGCGGCCAAGTACTATCAACAACAG
TTTGAAGAAGTACTCGTTGATGAATATCAAGATACAAACCTTGTGCAAGAAACGATTTTAAAGCTTGTATCTAAAGGGGA
ACATGCTGCAGAGGGCAACCTGTTTATGGTTGGTGATGTAAAGCAGTCCATATATCGTTTTAGACTGGCTGAGCCCATGC
TCTTTTTAAACAAATATAAACAATTTACACCAGACGGCAAAGAAACAGGGAAGAGAATTGATTTAAATAAAAACTTCCGC
AGCCGTTCAGATGTGTTAGACAGTACGAACTTTTTGTTCAAACAGCTGATGGGGGAAACAGTTGGAGAAATTGAATATGA
TGAACAAGCTGAATTAAAGCTTGGAGCGAGTTATCCAGAAAGCGAAGATACAACGACAGAAATGCTTCTTGTACATTTAG
ATCAACAGGAAGCAGAAACTGACGAAGAACGAGAAGAGCTAGAAACCGTACAGCTTGAAGCGAGAGTCATTGCTAAAAAA
ATAAGACAATTAGTGGAGCAGCCATTTCAAGTGTATGATTCAAAACAGCAAATGACCCGTAACTTGCAGTATCGGGACAT
TGTGATTTTGCTTCGCTCCATGCCGTGGGCACCGCAAATGATGGAGGAATTGAAGAAGCAGGGAATCCCTGTGTATGCGA
ATCTTTCCTCTGGTTATTTTGAAGCAACAGAGGTGTCTGTGATTCTTTCTTTGTTAAAAGTCATTGATAATCCATATCAA
GATATCCCGCTTGCAGCTGTATTAAGATCACCAATTGTTCATTTAGACGAAAATGAAATGGCACTGATCCGCACGAGTGA
TAAAAAAGGCACCTATTATGATGCTGTGAAAGCATTTATGAGTGTGACTCATTCAGATCACCCTACATGCAAAAAACTAG
AACGATTTTTCAGGTTGCTGCGTAAGTGGCGTGATTTCTCTATGAATCATTCAGTAGCGGAATTGATTTGGGAAGTATAC
CGCGATACGCAGTATCTAGATTACGTCGGCGGGATGCCTGGAGGCAAACAGAGACAAGCGAATCTCCGTGCTCTTTATGA
TCGAGCGAAGCAGTATGAAAAAGCTGCTTTTAGAGGGCTGTTCCGTTTTCTTCGCTTTATCGAAAGAATGCAGGAGCGTG
GAGATGATCTTGGGGCGGCAAAAACGTTCAGTGAAACAGAGGACGTTGTTCGCATGATGACGATCCACAGCAGTAAAGGA
TTAGAATTTCCAGTCGTCTTTACCGCAGGACTCGGCAGAAACTTTAATATGATGGACTTAAACCAGTCCTACTTACTTGA
TAAAGAATTAGGCTTTGGAAGCAAATATATTCATCCAGAGCTGAGAATTAGTTATGCAACATTACCGCTTGTTGCGATGA
AAAAAAAGATGAGGAAAGAGCTATTATCTGAAGAGCTGAGAGTTCTATATGTAGCGCTCACAAGGGCTAAGGAAAAATTA
TTTCTTGTCGGCTCTGTCAAAAATCAGGTGAAGGCATTAAGCAAATGGCAAAATGCTGCAACCGGAGAGGACTGGCTCCT
CCCTGATTTTGAACGTTATCAATCGAAAACATACTTAGATTTTATTGGTTCTGCTCTCATCCGTCACCAAGCAATGTCAT
CTATTTTAGAAGAAACAGGTGATGTTGTTCTTTCGCACCCATCAGCATTCACAATCTCATTTACTCAAGCTTCCGATCTT
CTAAAAGAAGATATGTCACTTGAAAAGAAGCAGCAAGATGAGGTGGTACAAGCATTGATGGACGGCTTACCTGTTGAAGG
ATATGGTGATGCTGATGAGAAAGTAGCAGAGAGACTTTCATGGAGATACCCGTATCTAGCGGCCTCACAAGTCGGGACAA
AGCATTCTGTTTCAGAGATAAAGAGAATGAAAGAAATACAGGATGAGTACAGTGTTCCTTCTTCTCTTAGAAGAGCTCAT
GCAACGTTGTATGACCGGCCGGCTTTCATGAAGAAAAAAGCACTCACTGCCGCAGAGCAAGGCACCGCAATGCATACAGT
CATGCAGCATATTCCGCTTTCTTCTGACGAGCCCCTCGATGAAACCCGTATACATCAGCTGCTCGATTCCTTACAGCAGC
GAGACTTGCTAACAAATGAGCAAGTTCAGTCAATTGATCAAGAAGGAATTTCCGCTTTTTTCTATACTTCTATTGGTCAA
AAGCTGCTGAAAGCGGACTGGGTGAAACGAGAAGTGTCATTTAGTATGGTGTTACCTGTTAAAGAGGTATACAGCCATAT
TGATGCAGAGGGGGAGCCTGTCCTAATTCAAGGGATGATTGACTGTTTATTTGAAACGGATGGAAAACTATATTTACTAG
ATTATAAAACAGATAGAGTGCAAGGCAGATATACAGGCGGTCTTGAAGCAGCTGTACCTATTTTGAAAAAGCGCTATGAA
ACTCAAATAGCGTTGTATGCACAAGCGGTAGAACGTTTAACAAACAGAACATTAGAGGAGAAAATTCTTTATTTCTTTGA
TGGGAATTTAGAAATTTCATTATAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-462)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1052-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (473-883)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	66.343	100	0.665