Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   KPF74_RS05505 Genome accession   NZ_CP076505
Coordinates   1069395..1070354 (+) Length   319 a.a.
NCBI ID   WP_002875425.1    Uniprot ID   -
Organism   Campylobacter jejuni strain R16.4752     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1049692..1079691 1069395..1070354 within 0


Gene organization within MGE regions


Location: 1049692..1079691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KPF74_RS05410 (KPF74_05410) pglI 1049692..1050621 (-) 930 WP_002890470.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  KPF74_RS05415 (KPF74_05415) pglH 1050614..1051693 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  KPF74_RS05420 (KPF74_05420) pglK 1051690..1053384 (-) 1695 WP_002890469.1 ABC-type lipopolysaccharide transporter PglK -
  KPF74_RS05425 (KPF74_05425) galE 1053378..1054364 (-) 987 WP_002853529.1 UDP-glucose 4-epimerase GalE -
  KPF74_RS05430 (KPF74_05430) - 1054422..1055216 (-) 795 WP_002853812.1 3'-5' exonuclease -
  KPF74_RS05435 (KPF74_05435) waaC 1055282..1056310 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  KPF74_RS05440 (KPF74_05440) htrB 1056300..1057187 (+) 888 WP_002890465.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  KPF74_RS05445 (KPF74_05445) - 1057184..1058731 (+) 1548 WP_002858148.1 glycosyltransferase -
  KPF74_RS05450 (KPF74_05450) - 1058735..1059907 (+) 1173 WP_002858013.1 glycosyltransferase family 2 protein -
  KPF74_RS05455 (KPF74_05455) - 1059891..1060886 (-) 996 WP_192802472.1 capsular polysaccharide synthesis protein -
  KPF74_RS05460 (KPF74_05460) - 1060944..1062113 (+) 1170 WP_002858320.1 glycosyltransferase family 2 protein -
  KPF74_RS05465 (KPF74_05465) wlaN 1062102..1063013 (-) 912 WP_002858039.1 beta-1,3 galactosyltransferase -
  KPF74_RS05470 (KPF74_05470) cstIII 1063069..1063953 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  KPF74_RS05475 (KPF74_05475) neuB1 1063934..1064965 (+) 1032 WP_002858213.1 sialic acid synthase -
  KPF74_RS05480 (KPF74_05480) neuC 1064962..1066077 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  KPF74_RS08925 (KPF74_05485) - 1066074..1066988 (+) 915 Protein_1074 beta-1,4-N-acetylgalactosaminyltransferase -
  KPF74_RS08930 (KPF74_05490) - 1067022..1067684 (+) 663 WP_002881429.1 N-acylneuraminate cytidylyltransferase -
  KPF74_RS05495 (KPF74_05495) - 1067679..1068528 (-) 850 Protein_1076 glycosyltransferase -
  KPF74_RS05500 (KPF74_05500) waaV 1068512..1069336 (-) 825 WP_002858096.1 glycosyltransferase family 2 protein -
  KPF74_RS05505 (KPF74_05505) waaF 1069395..1070354 (+) 960 WP_002875425.1 lipopolysaccharide heptosyltransferase II Regulator
  KPF74_RS05510 (KPF74_05510) gmhA 1070335..1070895 (-) 561 WP_002858021.1 D-sedoheptulose 7-phosphate isomerase -
  KPF74_RS05515 (KPF74_05515) rfaE1 1070892..1072277 (-) 1386 WP_002890180.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  KPF74_RS05520 (KPF74_05520) rfaD 1072270..1073223 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  KPF74_RS05525 (KPF74_05525) gmhB 1073224..1073784 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  KPF74_RS05530 (KPF74_05530) - 1073866..1074168 (+) 303 WP_002852762.1 cytochrome c -
  KPF74_RS05535 (KPF74_05535) ccoS 1074191..1074397 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  KPF74_RS05540 (KPF74_05540) - 1074394..1076751 (-) 2358 WP_072224466.1 heavy metal translocating P-type ATPase -
  KPF74_RS05545 (KPF74_05545) rho 1076860..1078158 (+) 1299 WP_002852852.1 transcription termination factor Rho -
  KPF74_RS05550 (KPF74_05550) dnaX 1078162..1079691 (+) 1530 WP_002875420.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=575879 KPF74_RS05505 WP_002875425.1 1069395..1070354(+) (waaF) [Campylobacter jejuni strain R16.4752]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=575879 KPF74_RS05505 WP_002875425.1 1069395..1070354(+) (waaF) [Campylobacter jejuni strain R16.4752]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

100

100

1