Detailed information NTG0575792

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: KPU58_RS05765
• Genome accession: NZ_CP076498
• Coordinates: 1125595..1127406 (+)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain UAMS_EL53
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	1115131..1134466	1125595..1127406	within	0

Gene organization within MGE regions

Location: 1115131..1134466

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KPU58_RS05715 (KPU58_05675)	trpS	1115724..1116734 (-)	1011	WP_002378619.1	tryptophan--tRNA ligase	-
KPU58_RS05720 (KPU58_05680)	-	1117088..1118281 (-)	1194	WP_010706472.1	site-specific integrase	-
KPU58_RS05725 (KPU58_05685)	-	1118360..1118563 (-)	204	WP_002607397.1	excisionase	-
KPU58_RS05730 (KPU58_05690)	-	1118563..1119624 (-)	1062	WP_256756141.1	replication initiation factor domain-containing protein	-
KPU58_RS05735 (KPU58_05695)	-	1120144..1120815 (+)	672	WP_002322214.1	helix-turn-helix transcriptional regulator	-
KPU58_RS05740 (KPU58_05700)	-	1121126..1121605 (+)	480	WP_002322212.1	MGMT family protein	-
KPU58_RS05745 (KPU58_05705)	dfrF	1121913..1122407 (+)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
KPU58_RS05750 (KPU58_05710)	spxA	1122877..1123275 (+)	399	WP_002356477.1	transcriptional regulator SpxA	-
KPU58_RS05755 (KPU58_05715)	-	1123617..1124279 (+)	663	WP_002356478.1	adaptor protein MecA	-
KPU58_RS05760 (KPU58_05720)	-	1124369..1125547 (+)	1179	WP_002387170.1	competence protein CoiA family protein	-
KPU58_RS05765 (KPU58_05725)	pepF	1125595..1127406 (+)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
KPU58_RS05770 (KPU58_05730)	-	1127498..1128157 (-)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
KPU58_RS05775 (KPU58_05735)	-	1128221..1128799 (-)	579	WP_002356483.1	CYTH domain-containing protein	-
KPU58_RS05780 (KPU58_05740)	-	1128996..1129670 (+)	675	WP_002356484.1	GTP pyrophosphokinase family protein	-
KPU58_RS05785 (KPU58_05745)	-	1129683..1130480 (+)	798	WP_002356486.1	NAD kinase	-
KPU58_RS05790 (KPU58_05750)	-	1130488..1131381 (+)	894	WP_002356487.1	RluA family pseudouridine synthase	-
KPU58_RS05795 (KPU58_05755)	mgtE	1131412..1132773 (+)	1362	WP_002362548.1	magnesium transporter	-
KPU58_RS05800 (KPU58_05760)	cutC	1132891..1133520 (-)	630	WP_002356490.1	copper homeostasis protein CutC	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=575792 KPU58_RS05765 WP_002387169.1 1125595..1127406(+) (pepF) [Enterococcus faecalis strain UAMS_EL53]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=575792 KPU58_RS05765 WP_002387169.1 1125595..1127406(+) (pepF) [Enterococcus faecalis strain UAMS_EL53]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539