Detailed information NTG0575748

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: KPU55_RS02595
• Genome accession: NZ_CP076493
• Coordinates: 515560..517371 (+)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain UAMS_EL54
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	505096..524431	515560..517371	within	0

Gene organization within MGE regions

Location: 505096..524431

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KPU55_RS02545 (KPU55_02530)	trpS	505689..506699 (-)	1011	WP_002378619.1	tryptophan--tRNA ligase	-
KPU55_RS02550 (KPU55_02535)	-	507053..508246 (-)	1194	WP_010706472.1	site-specific integrase	-
KPU55_RS02555 (KPU55_02540)	-	508325..508528 (-)	204	WP_002607397.1	excisionase	-
KPU55_RS02560 (KPU55_02545)	-	508528..509589 (-)	1062	WP_256756141.1	replication initiation factor domain-containing protein	-
KPU55_RS02565 (KPU55_02550)	-	510109..510780 (+)	672	WP_002322214.1	helix-turn-helix transcriptional regulator	-
KPU55_RS02570 (KPU55_02555)	-	511091..511570 (+)	480	WP_002322212.1	MGMT family protein	-
KPU55_RS02575 (KPU55_02560)	dfrF	511878..512372 (+)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
KPU55_RS02580 (KPU55_02565)	spxA	512842..513240 (+)	399	WP_002356477.1	transcriptional regulator SpxA	-
KPU55_RS02585 (KPU55_02570)	-	513582..514244 (+)	663	WP_002356478.1	adaptor protein MecA	-
KPU55_RS02590 (KPU55_02575)	-	514334..515512 (+)	1179	WP_002387170.1	competence protein CoiA family protein	-
KPU55_RS02595 (KPU55_02580)	pepF	515560..517371 (+)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
KPU55_RS02600 (KPU55_02585)	-	517463..518122 (-)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
KPU55_RS02605 (KPU55_02590)	-	518186..518764 (-)	579	WP_002356483.1	CYTH domain-containing protein	-
KPU55_RS02610 (KPU55_02595)	-	518961..519635 (+)	675	WP_002356484.1	GTP pyrophosphokinase family protein	-
KPU55_RS02615 (KPU55_02600)	-	519648..520445 (+)	798	WP_002356486.1	NAD kinase	-
KPU55_RS02620 (KPU55_02605)	-	520453..521346 (+)	894	WP_002356487.1	RluA family pseudouridine synthase	-
KPU55_RS02625 (KPU55_02610)	mgtE	521377..522738 (+)	1362	WP_002362548.1	magnesium transporter	-
KPU55_RS02630 (KPU55_02615)	cutC	522856..523485 (-)	630	WP_002356490.1	copper homeostasis protein CutC	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=575748 KPU55_RS02595 WP_002387169.1 515560..517371(+) (pepF) [Enterococcus faecalis strain UAMS_EL54]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=575748 KPU55_RS02595 WP_002387169.1 515560..517371(+) (pepF) [Enterococcus faecalis strain UAMS_EL54]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539