Detailed information NTG0575720

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: KPU60_RS04915
• Genome accession: NZ_CP076488
• Coordinates: 964192..966003 (+)
• Length: 603 a.a.
• NCBI ID: WP_002387169.1
• Uniprot ID: A0A855UCR5
• Organism: Enterococcus faecalis strain UAMS_EL56
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
ICE	953728..973063	964192..966003	within	0

Gene organization within MGE regions

Location: 953728..973063

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KPU60_RS04865 (KPU60_04830)	trpS	954321..955331 (-)	1011	WP_002378619.1	tryptophan--tRNA ligase	-
KPU60_RS04870 (KPU60_04835)	-	955685..956878 (-)	1194	WP_010706472.1	site-specific integrase	-
KPU60_RS04875 (KPU60_04840)	-	956957..957160 (-)	204	WP_002607397.1	excisionase	-
KPU60_RS04880 (KPU60_04845)	-	957160..958221 (-)	1062	WP_256756141.1	replication initiation factor domain-containing protein	-
KPU60_RS04885 (KPU60_04850)	-	958741..959412 (+)	672	WP_002322214.1	helix-turn-helix transcriptional regulator	-
KPU60_RS04890 (KPU60_04855)	-	959723..960202 (+)	480	WP_002322212.1	MGMT family protein	-
KPU60_RS04895 (KPU60_04860)	dfrF	960510..961004 (+)	495	WP_002322211.1	trimethoprim-resistant dihydrofolate reductase DfrF	-
KPU60_RS04900 (KPU60_04865)	spxA	961474..961872 (+)	399	WP_002356477.1	transcriptional regulator SpxA	-
KPU60_RS04905 (KPU60_04870)	-	962214..962876 (+)	663	WP_002356478.1	adaptor protein MecA	-
KPU60_RS04910 (KPU60_04875)	-	962966..964144 (+)	1179	WP_002387170.1	competence protein CoiA family protein	-
KPU60_RS04915 (KPU60_04880)	pepF	964192..966003 (+)	1812	WP_002387169.1	oligoendopeptidase F	Regulator
KPU60_RS04920 (KPU60_04885)	-	966095..966754 (-)	660	WP_002368840.1	ClpXP adapter SpxH family protein	-
KPU60_RS04925 (KPU60_04890)	-	966818..967396 (-)	579	WP_002356483.1	CYTH domain-containing protein	-
KPU60_RS04930 (KPU60_04895)	-	967593..968267 (+)	675	WP_002356484.1	GTP pyrophosphokinase family protein	-
KPU60_RS04935 (KPU60_04900)	-	968280..969077 (+)	798	WP_002356486.1	NAD kinase	-
KPU60_RS04940 (KPU60_04905)	-	969085..969978 (+)	894	WP_002356487.1	RluA family pseudouridine synthase	-
KPU60_RS04945 (KPU60_04910)	mgtE	970009..971370 (+)	1362	WP_002362548.1	magnesium transporter	-
KPU60_RS04950 (KPU60_04915)	cutC	971488..972117 (-)	630	WP_002356490.1	copper homeostasis protein CutC	-

Sequence

Protein

• Length: 603 a.a.
• Molecular weight: 68848.16 Da
• Isoelectric Point: 4.6861

>NTDB_id=575720 KPU60_RS04915 WP_002387169.1 964192..966003(+) (pepF) [Enterococcus faecalis strain UAMS_EL56]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLIEELNQAESFKGTLGDGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAGEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide

• Length: 1812 bp

>NTDB_id=575720 KPU60_RS04915 WP_002387169.1 964192..966003(+) (pepF) [Enterococcus faecalis strain UAMS_EL56]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGATAGAAGAGCTAAATCAAGCAGAATCATTTAAAGGTACCTTAGGGG
ATGGTGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAGTTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGTTTGTACAAAGTATACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACACATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGAGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGCTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACAAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCACATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGGAGAAGAGGGAGCTTTAGAGAATTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACACAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (207-587)

• Oligopeptidase F (Peptidase_M3_N) (117-186)

3D structure

• AlphaFold DB: A0A855UCR5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	54.806	98.342	0.539