Detailed information NTG0575501

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KOM03_RS17425
• Genome accession: NZ_CP076450
• Coordinates: 3569842..3570456 (-)
• Length: 204 a.a.
• NCBI ID: WP_060562641.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GMEKP1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3564842..3575456

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KOM03_RS17395 (KOM03_17395)	rpsT	3565141..3565407 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KOM03_RS17400 (KOM03_17400)	holA	3565424..3566469 (-)	1046	Protein_3401	DNA polymerase III subunit delta	-
KOM03_RS17405 (KOM03_17405)	-	3566509..3566660 (-)	152	Protein_3402	hypothetical protein	-
KOM03_RS17410 (KOM03_17410)	-	3566701..3566835 (+)	135	WP_003152870.1	YqzM family protein	-
KOM03_RS17415 (KOM03_17415)	comEC	3566854..3569205 (-)	2352	WP_060562640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KOM03_RS17420 (KOM03_17420)	-	3569206..3569775 (-)	570	WP_003152868.1	ComE operon protein 2	-
KOM03_RS17425 (KOM03_17425)	comEA	3569842..3570456 (-)	615	WP_060562641.1	helix-hairpin-helix domain-containing protein	Machinery gene
KOM03_RS17430 (KOM03_17430)	comER	3570515..3571336 (+)	822	WP_216119320.1	late competence protein ComER	-
KOM03_RS17435 (KOM03_17435)	-	3571405..3572142 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
KOM03_RS17440 (KOM03_17440)	rsfS	3572139..3572495 (-)	357	WP_007408260.1	ribosome silencing factor	-
KOM03_RS17445 (KOM03_17445)	yqeK	3572513..3573073 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KOM03_RS17450 (KOM03_17450)	-	3573063..3573632 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
KOM03_RS17455 (KOM03_17455)	yhbY	3573643..3573933 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KOM03_RS17460 (KOM03_17460)	aroE	3573927..3574769 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22050.22 Da
• Isoelectric Point: 6.4803

>NTDB_id=575501 KOM03_RS17425 WP_060562641.1 3569842..3570456(-) (comEA) [Bacillus velezensis strain GMEKP1]
MTKAMMTERLLRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLGELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=575501 KOM03_RS17425 WP_060562641.1 3569842..3570456(-) (comEA) [Bacillus velezensis strain GMEKP1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCTGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTATAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGGCGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae R6	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.377	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.377	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.377	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	34.906	100	0.363