Detailed information    

experimental Experimentally validated

Overview

Name   comEC/comE3   Type   Machinery gene
Locus tag   C694_RS07015 Genome accession   NC_018939
Coordinates   1421624..1422937 (-) Length   437 a.a.
NCBI ID   WP_000653622.1    Uniprot ID   -
Organism   Helicobacter pylori 26695     
Function   ssDNA transport through the inner membrane   
DNA binding and uptake

Function

HP1361 is a comE3 homologue and is required for DNA binding and uptake during DNA transformation.


Genomic Context

Location: 1416624..1427937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS06985 (C694_06995) nadC 1417087..1417908 (-) 822 WP_000405980.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C694_RS06990 (C694_07000) nadA 1417908..1418918 (-) 1011 WP_001141770.1 quinolinate synthase NadA -
  C694_RS06995 (C694_07005) - 1418908..1419711 (-) 804 WP_000226149.1 phosphatidylserine decarboxylase -
  C694_RS07000 (C694_07010) - 1419705..1420211 (-) 507 WP_000953192.1 DUF6115 domain-containing protein -
  C694_RS07005 (C694_07015) - 1420224..1420718 (-) 495 WP_001862592.1 hypothetical protein -
  C694_RS07010 (C694_07020) mqnP 1420711..1421553 (-) 843 WP_229311982.1 menaquinone biosynthesis prenyltransferase MqnP -
  C694_RS07015 (C694_07025) comEC/comE3 1421624..1422937 (-) 1314 WP_000653622.1 ComEC/Rec2 family competence protein Machinery gene
  C694_RS07020 (C694_07030) - 1422934..1424400 (-) 1467 WP_000349722.1 replicative DNA helicase -
  C694_RS07025 (C694_07035) - 1424411..1425811 (-) 1401 WP_000954017.1 NAD(P)H-hydrate dehydratase -
  C694_RS07030 (C694_07040) crdS 1425814..1427007 (-) 1194 WP_000951935.1 copper-sensing histidine kinase CrdS -
  C694_RS07035 (C694_07045) crdR 1426982..1427623 (-) 642 WP_001169789.1 copper response regulator transcription factor CrdR -

Sequence

Protein


Download         Length: 437 a.a.        Molecular weight: 50295.30 Da        Isoelectric Point: 9.8514

>NTDB_id=1208 C694_RS07015 WP_000653622.1 1421624..1422937(-) (comEC/comE3) [Helicobacter pylori 26695]
MKDKTFQGAFELLTTPKEYLVCGVFLSLLLAINLYLEYLNYQKLDFSKPTSLSAQILLQYPKTKDQKTYFVLKLQSKNMI
FYTTIKEPLKNLQYRHAQFFGKIKPCSFLESLKSCFFQTYSFSLTRKQDFKSHWRHFIDSAHENALVGNLYRALFIGDSL
NKDLRDRANALGINHLLAISGFHLGILSVSVYFLSSLFYTPLQKRYFPYRNAFYDIGVLVWVFLLGYLLLLDFLPSFFRA
FLMGLLGFLACFFGVRLLSFKLLILACCIAIALLPKLLFSVGFLLSVCGVWYIFLFLKHTQIFFKTSSFLMRSFQAISLS
ALVFLNMLIIVHAFFPMFSPYQLFSIPLGLIFIVFFPLSLFLHAVGLGSLLDRLLSMPLTIPTISVPSPLWLLGVHLFLT
ILSARFFKVYLSMNVLSAGFFLYCCYQYIIMPSLIVG

Nucleotide


Download         Length: 1314 bp        

>NTDB_id=1208 C694_RS07015 WP_000653622.1 1421624..1422937(-) (comEC/comE3) [Helicobacter pylori 26695]
TTGAAAGACAAAACTTTTCAGGGGGCGTTTGAACTTCTTACGACCCCCAAAGAATACCTGGTGTGTGGGGTGTTTTTAAG
CCTTTTATTGGCGATTAACCTTTATTTAGAATACTTGAATTACCAAAAGCTTGATTTTTCAAAACCTACAAGTTTGAGCG
CTCAAATCTTGTTGCAATACCCTAAAACTAAAGATCAAAAAACCTATTTTGTTTTAAAGCTCCAATCAAAAAACATGATC
TTTTACACCACCATTAAAGAGCCTTTAAAAAACCTCCAATACCGCCATGCGCAATTTTTTGGCAAAATCAAACCTTGCTC
GTTCTTAGAGTCTCTAAAATCATGCTTTTTTCAAACTTATTCTTTTTCTTTAACACGAAAACAGGATTTCAAATCGCATT
GGCGCCATTTCATTGACAGCGCTCATGAAAACGCTTTGGTGGGTAATTTATATCGCGCGTTATTTATAGGGGATAGCTTA
AATAAAGACTTAAGGGATAGGGCTAACGCGCTAGGGATCAACCACTTACTAGCCATTAGCGGGTTCCATTTAGGGATTTT
GAGCGTTAGCGTGTATTTTCTTTCCTCTCTTTTTTATACCCCCTTACAAAAACGCTATTTCCCTTATAGGAACGCTTTTT
ATGATATAGGGGTTTTGGTGTGGGTTTTTTTGCTGGGGTATTTGTTGCTACTAGATTTTTTACCCTCTTTTTTCAGGGCG
TTTTTAATGGGCTTATTAGGGTTTTTGGCATGCTTTTTTGGGGTAAGGCTTTTGAGTTTTAAACTTTTGATTTTAGCGTG
CTGTATCGCCATAGCGTTACTCCCTAAATTGCTTTTTAGCGTGGGGTTTTTGCTTTCTGTTTGTGGGGTGTGGTATATCT
TCTTGTTTTTAAAACACACTCAAATTTTTTTTAAAACCTCTTCTTTTTTGATGCGATCTTTTCAAGCCATAAGCTTAAGC
GCGCTGGTGTTTTTGAACATGCTCATTATCGTGCATGCCTTTTTCCCTATGTTTTCGCCCTACCAGCTCTTTAGCATTCC
TTTAGGCTTGATTTTTATCGTGTTTTTCCCTTTGAGTTTGTTCTTGCATGCGGTGGGTTTGGGGTCTTTGTTGGATCGCC
TTTTAAGCATGCCTTTAACAATCCCTACGATTTCGGTTCCTTCGCCCTTATGGCTTTTAGGGGTGCATTTATTTTTAACG
ATTTTAAGCGCGCGTTTTTTTAAAGTTTATTTAAGCATGAATGTTTTAAGTGCGGGCTTTTTCTTGTATTGTTGCTATCA
ATATATTATAATGCCTAGCTTAATTGTAGGTTAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(159-371)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Prashant P Damke et al. (2019) Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori. Nature Communications 10(1):5357. [PMID: 31767852]
[2] Yu-Ching Yeh et al. (2003) Characterization of a ComE3 homologue essential for DNA transformation in Helicobacter pylori. Infection And Immunity 71(9):5427-31. [PMID: 12933898]