Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KOM03_RS05705 Genome accession   NZ_CP076450
Coordinates   1113835..1115214 (+) Length   459 a.a.
NCBI ID   WP_014416742.1    Uniprot ID   I2C0L1
Organism   Bacillus velezensis strain GMEKP1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1108835..1120214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOM03_RS05685 (KOM03_05685) ctsR 1109186..1109650 (+) 465 WP_003156396.1 transcriptional regulator CtsR -
  KOM03_RS05690 (KOM03_05690) - 1109664..1110221 (+) 558 WP_060562029.1 UvrB/UvrC motif-containing protein -
  KOM03_RS05695 (KOM03_05695) - 1110221..1111312 (+) 1092 WP_003156398.1 protein arginine kinase -
  KOM03_RS05700 (KOM03_05700) clpC 1111309..1113741 (+) 2433 WP_060562030.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  KOM03_RS05705 (KOM03_05705) radA 1113835..1115214 (+) 1380 WP_014416742.1 DNA repair protein RadA Machinery gene
  KOM03_RS05710 (KOM03_05710) disA 1115218..1116300 (+) 1083 WP_007615225.1 DNA integrity scanning diadenylate cyclase DisA -
  KOM03_RS05715 (KOM03_05715) - 1116416..1117516 (+) 1101 WP_003156403.1 PIN/TRAM domain-containing protein -
  KOM03_RS05720 (KOM03_05720) ispD 1117529..1118226 (+) 698 Protein_1098 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KOM03_RS05725 (KOM03_05725) ispF 1118219..1118695 (+) 477 WP_003156407.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49547.98 Da        Isoelectric Point: 7.1760

>NTDB_id=575456 KOM03_RS05705 WP_014416742.1 1113835..1115214(+) (radA) [Bacillus velezensis strain GMEKP1]
MAKSKTKFICHSCGYESAKWMGKCPGCGAWNTMVEETIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVHTKLEEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLADTAGSVLYISGEESVKQTKLRADRLGINSQMLHVLSETDMEYIS
SAIQEMKPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTNGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGASGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPEANADGWTIPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=575456 KOM03_RS05705 WP_014416742.1 1113835..1115214(+) (radA) [Bacillus velezensis strain GMEKP1]
ATGGCTAAATCAAAAACAAAATTCATCTGCCACTCATGCGGTTATGAATCCGCAAAATGGATGGGAAAGTGCCCGGGCTG
CGGGGCTTGGAATACAATGGTGGAAGAAACCATAAAAAAAGCGCCGGCGAATCGGCGGGCGGCTTTCTCCCACTCCGTTC
AAACCGTCCAGAAACCATCACCCATTACATCAATTGAAACATCGGAAGAACCCCGCGTTCACACAAAGCTTGAAGAATTC
AACAGAGTGCTTGGCGGCGGCGTTGTGAAGGGCTCGTTAGTATTAATCGGCGGCGATCCCGGGATCGGAAAATCAACGCT
CCTTCTCCAAGTATCCGCACAATTAGCAGATACGGCCGGCAGTGTGCTTTACATTTCAGGGGAAGAATCCGTGAAGCAAA
CGAAGCTGCGCGCCGACAGACTTGGAATCAACAGCCAAATGTTACATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAAACCCGCATTTGTTGTCGTAGACTCCATTCAGACCGTTTATCAAAGTGATATCACCTCGGC
TCCCGGCAGTGTATCACAAGTAAGAGAATGTACGGCTGAATTGATGAAAATCGCGAAAACAAACGGCATACCTATTTTTA
TCGTCGGGCACGTCACAAAAGAAGGATCGATCGCGGGTCCGAGATTGCTTGAGCACATGGTCGATACCGTTCTATACTTT
GAAGGAGAGCGGCACCATACATTCCGTATTTTGCGGGCCGTAAAAAACCGTTTTGGCTCAACAAATGAAATGGGTATCTT
TGAAATGCGGGAAGAGGGGCTTACGGAAGTGCTGAACCCTTCTGAAATTTTCTTGGAAGAGCGTTCTGCCGGGGCATCGG
GTTCGAGTATCGTTGCGTCAATGGAAGGAACGAGACCGATCCTGGTTGAAATTCAGGCGCTCATATCACCGACCAGTTTT
GGAAACCCGCGCCGCATGGCTACAGGCATTGACCACAACAGGGTATCGTTAATTATGGCTGTGCTTGAGAAGCGGGTGGG
TCTTTTGCTTCAAAATCAAGATGCGTATTTAAAGGTCGCCGGCGGGGTCAAACTGGATGAACCGGCGATTGATTTGGCAG
TAGCCGTCAGCATTGCCTCAAGCTTCAGAGATACACCGCCGAATCCCGCAGATTGCTTTATCGGTGAAGTGGGTCTGACA
GGAGAAGTCCGCAGGGTGTCAAGAATCGAACAGCGCGTAAAAGAAGCGGCAAAACTCGGCTTCAAGCGCATGATCATACC
TGAGGCGAATGCAGATGGGTGGACAATACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(73-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C0L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

93.45

99.782

0.932
  radA Streptococcus pneumoniae Rx1

63.797

98.693

0.63
  radA Streptococcus pneumoniae D39

63.797

98.693

0.63
  radA Streptococcus pneumoniae R6

63.797

98.693

0.63
  radA Streptococcus pneumoniae TIGR4

63.797

98.693

0.63
  radA Streptococcus mitis NCTC 12261

63.797

98.693

0.63
  radA Streptococcus mitis SK321

63.576

98.693

0.627