Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KMZ16_RS01975 Genome accession   NZ_CP076445
Coordinates   370392..370841 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis subsp. subtilis strain ps4100     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 365392..375841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ16_RS01940 (KMZ16_01935) yckA 365543..366223 (-) 681 WP_003234624.1 amino acid ABC transporter permease -
  KMZ16_RS01945 (KMZ16_01940) yckB 366233..367096 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  KMZ16_RS01950 (KMZ16_01945) - 367258..367455 (+) 198 WP_119122841.1 hypothetical protein -
  KMZ16_RS01955 (KMZ16_01950) yckC 367474..367929 (+) 456 WP_003246356.1 RDD family protein -
  KMZ16_RS01960 (KMZ16_01955) yckD 368011..368343 (+) 333 WP_003246247.1 YckD family protein -
  KMZ16_RS01965 (KMZ16_01960) bglC 368497..369930 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  KMZ16_RS01970 (KMZ16_01965) nin/comJ 369967..370365 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  KMZ16_RS01975 (KMZ16_01970) nucA/comI 370392..370841 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  KMZ16_RS01980 (KMZ16_01975) tlpC 371009..372730 (-) 1722 WP_029727077.1 methyl-accepting chemotaxis protein TlpC -
  KMZ16_RS01985 (KMZ16_01980) hxlB 372841..373398 (-) 558 WP_003234605.1 6-phospho-3-hexuloisomerase -
  KMZ16_RS01990 (KMZ16_01985) hxlA 373404..374036 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  KMZ16_RS01995 (KMZ16_01990) hxlR 374269..374631 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=575310 KMZ16_RS01975 WP_003234608.1 370392..370841(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ps4100]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=575310 KMZ16_RS01975 WP_003234608.1 370392..370841(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ps4100]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98