Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KMZ15_RS04270 Genome accession   NZ_CP076444
Coordinates   1032636..1034018 (+) Length   460 a.a.
NCBI ID   WP_223694529.1    Uniprot ID   -
Organism   Mycoavidus sp. HKI     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1027636..1039018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KMZ15_RS04250 (KMZ15_04220) - 1028015..1028938 (-) 924 WP_223694519.1 Rpn family recombination-promoting nuclease/putative transposase -
  KMZ15_RS08975 - 1029146..1029349 (+) 204 WP_223694521.1 hypothetical protein -
  KMZ15_RS04255 (KMZ15_04230) thiD 1029282..1030088 (-) 807 WP_223694523.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  KMZ15_RS04260 (KMZ15_04235) lplT 1030123..1031427 (-) 1305 WP_223694525.1 lysophospholipid transporter LplT -
  KMZ15_RS04265 (KMZ15_04240) alr 1031564..1032634 (+) 1071 WP_223694527.1 alanine racemase -
  KMZ15_RS04270 (KMZ15_04245) radA 1032636..1034018 (+) 1383 WP_223694529.1 DNA repair protein RadA Machinery gene
  KMZ15_RS04275 (KMZ15_04250) - 1034129..1034389 (-) 261 WP_223694531.1 hypothetical protein -
  KMZ15_RS04280 (KMZ15_04255) - 1034386..1034733 (-) 348 WP_223694533.1 holin -
  KMZ15_RS04285 (KMZ15_04260) - 1035026..1036441 (+) 1416 WP_223694535.1 MFS transporter -
  KMZ15_RS04290 (KMZ15_04265) - 1036592..1036735 (+) 144 Protein_827 recombinase family protein -
  KMZ15_RS04295 (KMZ15_04270) - 1036837..1038765 (-) 1929 WP_223694537.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 48969.69 Da        Isoelectric Point: 8.1113

>NTDB_id=575300 KMZ15_RS04270 WP_223694529.1 1032636..1034018(+) (radA) [Mycoavidus sp. HKI]
MAKKTLYACGVCGAQSPKWQGQCLACNAWNSLIETVAEAPATHRYQALAKTAPVQKLATIEAADVPRFSSGIQEFDRVLG
GGLVAGGVVLIGGDPGIGKSTLLLQSLAVLARERRALYVSGEESSAQIALRAQRLGLMESTAPASELHLLAEIQLEKIQA
AITAERPAVAVIDSIQTVYSEALSSAPGSVAQVRECAAQLTRLAKQMGVTIIMVGHVTKEGSLAGPRVLEHIVDTVLYFE
GDTHSAYRLVRAFKNRFGAVNELGVFAMTERGLRGVANPSALFLSQHEHRVPGSCVLVTQEGSRPLLVEIQALVDTAHIP
NPRRLAVGLEQNRLAMLLAVLNRHAGIACFDQDVFLNAVGGVKISEPAADLAVLLAIQSSLRNKPLPNGLVVFGEIGLAG
EIRPCPRGQERLKEAAKLGFSMALIPAANAPKQPIEGLEIIAVERIEKAIEQIRARNNNS

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=575300 KMZ15_RS04270 WP_223694529.1 1032636..1034018(+) (radA) [Mycoavidus sp. HKI]
ATGGCGAAAAAAACACTTTATGCCTGTGGTGTTTGTGGCGCGCAGTCGCCCAAATGGCAAGGGCAGTGTCTTGCCTGCAA
TGCATGGAATTCACTGATTGAGACCGTGGCTGAAGCACCCGCCACCCACCGCTATCAAGCGCTAGCCAAGACCGCTCCGG
TGCAAAAACTGGCGACCATCGAGGCCGCCGATGTGCCACGCTTTTCAAGCGGCATTCAAGAGTTTGACCGGGTGCTTGGT
GGTGGTTTAGTGGCAGGTGGGGTGGTCCTAATTGGCGGAGACCCGGGCATTGGTAAATCAACCCTGTTATTGCAGTCGCT
TGCCGTCTTGGCCCGTGAGCGCCGCGCGCTTTATGTGAGCGGCGAAGAGTCGTCTGCACAAATCGCCTTGCGGGCGCAGC
GCTTGGGTCTAATGGAAAGCACGGCGCCCGCAAGCGAACTGCATTTGCTGGCCGAGATTCAGCTCGAGAAAATACAAGCA
GCAATTACTGCTGAACGGCCGGCAGTGGCGGTTATTGATTCGATCCAAACCGTCTATTCAGAGGCGCTGAGCTCAGCGCC
TGGCTCAGTTGCCCAAGTCCGCGAATGTGCCGCGCAACTCACGCGCTTAGCCAAGCAAATGGGCGTGACTATCATTATGG
TCGGCCATGTGACCAAAGAAGGCAGTCTGGCCGGGCCGCGGGTGCTGGAACATATTGTTGACACAGTGCTGTATTTTGAG
GGGGATACTCATTCCGCCTACCGGCTGGTGCGGGCCTTTAAAAATCGCTTTGGCGCAGTCAATGAGCTCGGGGTGTTTGC
GATGACCGAGCGCGGTTTGCGCGGGGTGGCGAACCCCTCTGCGCTTTTTCTATCACAACATGAACACAGAGTGCCCGGCT
CTTGTGTATTAGTGACCCAAGAAGGCTCGCGGCCATTATTGGTTGAGATTCAGGCCTTAGTCGACACCGCTCATATACCC
AATCCCCGTCGACTCGCAGTGGGGCTTGAGCAAAATCGGTTGGCGATGTTATTGGCGGTATTAAACCGGCATGCAGGCAT
TGCGTGTTTTGATCAAGACGTGTTTTTAAATGCCGTAGGGGGCGTCAAAATCTCTGAGCCAGCCGCGGATCTTGCAGTGT
TGCTGGCAATTCAATCCTCCCTGCGCAACAAACCGTTGCCTAACGGCTTAGTGGTATTTGGCGAAATTGGGCTGGCTGGC
GAAATCCGCCCCTGCCCACGCGGACAAGAGCGCTTGAAGGAAGCGGCCAAGCTTGGTTTTTCAATGGCTTTGATTCCAGC
CGCAAACGCCCCTAAGCAGCCGATTGAAGGGCTTGAAATAATCGCTGTTGAGCGGATTGAAAAAGCGATTGAACAGATAC
GGGCACGCAATAATAATTCATGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(362-452)

AAA domain (AAA_25)

(64-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.567

100

0.498
  radA Streptococcus mitis SK321

46.608

99.348

0.463
  radA Streptococcus pneumoniae Rx1

46.304

100

0.463
  radA Streptococcus pneumoniae D39

46.304

100

0.463
  radA Streptococcus pneumoniae R6

46.304

100

0.463
  radA Streptococcus pneumoniae TIGR4

46.304

100

0.463
  radA Streptococcus mitis NCTC 12261

46.272

99.13

0.459