Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KOL96_RS09565 Genome accession   NZ_CP076413
Coordinates   334501..336009 (-) Length   502 a.a.
NCBI ID   WP_232041887.1    Uniprot ID   A0AAD2EGZ9
Organism   Ralstonia wenshanensis strain 56D2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 329501..341009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOL96_RS09535 (KOL96_09470) - 329973..330626 (-) 654 WP_232041884.1 LON peptidase substrate-binding domain-containing protein -
  KOL96_RS09540 (KOL96_09475) rapZ 330730..331623 (-) 894 WP_232041885.1 RNase adapter RapZ -
  KOL96_RS09545 (KOL96_09480) - 331718..332059 (-) 342 WP_232041886.1 PsiF family protein -
  KOL96_RS09550 (KOL96_09485) hprK 332246..333220 (-) 975 WP_004633393.1 HPr(Ser) kinase/phosphatase -
  KOL96_RS09555 (KOL96_09490) ptsN 333294..333749 (-) 456 WP_012761097.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KOL96_RS09560 (KOL96_09495) raiA 334097..334450 (-) 354 WP_004633388.1 ribosome-associated translation inhibitor RaiA -
  KOL96_RS09565 (KOL96_09500) rpoN/rpoN1 334501..336009 (-) 1509 WP_232041887.1 RNA polymerase factor sigma-54 Machinery gene
  KOL96_RS09570 (KOL96_09505) lptB 336106..336894 (-) 789 WP_232041888.1 LPS export ABC transporter ATP-binding protein -
  KOL96_RS09575 (KOL96_09510) lptA 336925..337515 (-) 591 WP_232041889.1 lipopolysaccharide transport periplasmic protein LptA -
  KOL96_RS09580 (KOL96_09515) lptC 337575..338207 (-) 633 WP_045202514.1 LPS export ABC transporter periplasmic protein LptC -
  KOL96_RS09585 (KOL96_09520) - 338212..338808 (-) 597 WP_232041890.1 HAD family hydrolase -
  KOL96_RS09590 (KOL96_09525) - 338825..339808 (-) 984 WP_232043085.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55618.00 Da        Isoelectric Point: 4.8510

>NTDB_id=575177 KOL96_RS09565 WP_232041887.1 334501..336009(-) (rpoN/rpoN1) [Ralstonia wenshanensis strain 56D2]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSNSATPLPTE
SPPPAPNGGDSSDTPDSYENSHDNGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLKL
SMRDKGLVIFLIESLDDEGYLAGTLDEILADLPAELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLDSPAKA
LALIIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVIVRKTSAGWMAQLNPDVM
PRLRINDMYAQILRSSRGESGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTL
GLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=575177 KOL96_RS09565 WP_232041887.1 334501..336009(-) (rpoN/rpoN1) [Ralstonia wenshanensis strain 56D2]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCCCAGCATCTCGCGCTCACGCCGCAGTTGCAGCAATCGATCCGATTGCTGCA
GTTGTCGACCATAGAACTGCAGCAAGAAGTTGAGCAGGCCCTGACTGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACCTCCGCACGCATCGGCGCGGACGGATCAGTGAACGCGTTGCAAAGCAACAGCGCCACGCCCCTGCCCACCGAA
TCGCCTCCGCCCGCACCCAACGGTGGCGACAGCAGCGACACCCCCGACAGCTACGAGAACAGTCACGACAACGGCGCCGA
TTTCGACAGCGATTACGGTTCCGACCGCTCCGACTGGAGCCTTGACGACTTCGCCCGCAAACCGCAATCCGACGAAGACG
ACCGCGCCCCGCTGCAATTGCGCGAGGCCGAACAAAGCCTGCGCGAATTCCTCATGGAACAGCTCGCACCGCTCAAGCTG
TCGATGCGCGACAAGGGCCTCGTCATTTTCCTGATCGAGTCGCTCGACGACGAAGGCTATCTGGCAGGCACACTCGACGA
AATCCTCGCGGATCTGCCTGCCGAACTCGAAGTCGAACTCGACGAACTGCAAGCCGCGCTACGCATGCTGCAGAGTTTTG
ATCCGCCTGGCATTGGCGCACGCTCAGCCGCCGAGTGCCTGTCACTGCAATTGCACCGGCTCGATTCGCCCGCGAAGGCG
CTGGCGCTCATCATCGTCAATCAGCATCTCGAACTGCTGGCTGCGCGTGACTACACGCGCCTGAAGAAGGCACTGTCCGT
CGACGAACCCGCACTGAAAGCAGCGCATGAGCTGATCCGTTCACTGGCACCGTTCCCGGGTCATGCGTTCGGTCGCGCCG
AGGCGGATTTCGTAGTGCCTGACGTGATCGTGCGCAAGACGAGCGCAGGCTGGATGGCGCAACTGAATCCCGATGTGATG
CCCCGCCTGCGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGTGAATCGGGCGCCGCCAATCTACAGCA
GAAGCTGCAGGAAGCACGTTGGCTGATCAAGAACATCCAGCAGCGCTTCGATACGATCCTGCGTGTCTCGCAGGCCATTG
TCGAGCGTCAAAAGAGCTTCTTCACGCACGGTGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTG
GGTCTACACGAGTCCACGATCTCCCGTGTGACAACCAACAAATACATGGCGACCCCGATGGGGACGTTCGAGTTGAAGTA
CTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCTGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTG
TAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCC
CGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

93.625

100

0.936